Diagnostic Laboratory of Oklahoma, 225 NE 97th Street, Oklahoma City, OK 73114 United States.
Immunohematology. 2022 Sep 22;38(3):90-95. doi: 10.21307/immunohematology-2022-048. Print 2022 Sep 1.
Proteolytic enzymes are used to treat red blood cells (RBCs) to aid in complex antibody identification. Although there are many enzymes that can be used, for the purpose of this method review, enzyme-treated RBCs refers only to RBCs treated with ficin or papain. Ficin and papain can increase the sensitivity of antibody detection by modifying the RBC membrane. Enzyme treatment and test methods can be performed using one-stage or two-stage procedures. Enzyme treatment is especially useful for the differentiation of multiple antibodies, enhancement of detection of weak antibodies, and adsorption methods. In all cases, quality control is required to ensure adequate treatment of RBCs before additional testing. Ficin and papain are useful tools for both immunohematology reference laboratories and transfusion services.
蛋白酶用于处理红细胞 (RBC) 以帮助复杂抗体的鉴定。尽管有许多可以使用的酶，但就本方法综述而言，酶处理的 RBC 仅指用ficin 或木瓜蛋白酶处理的 RBC。ficin 和木瓜蛋白酶可以通过修饰 RBC 膜来提高抗体检测的灵敏度。酶处理和测试方法可以使用一步法或两步法进行。酶处理对于区分多种抗体、增强弱抗体的检测以及吸附方法特别有用。在所有情况下，都需要质量控制以确保在进行额外的测试之前充分处理 RBC。ficin 和木瓜蛋白酶是免疫血液学参考实验室和输血服务的有用工具。
