ZZAP对红细胞的处理

ZZAP treatment of red blood cells.

作者信息

Marckwardt Sarah I

机构信息

IRL Technologist III, Immunohematology Reference Laboratory, American Red Cross Southeastern Michigan Region.

出版信息

Immunohematology. 2019 Jan;35(1):9-10.

PMID:30908071

Abstract

ZZAP is a mixture of a sulfhydryl reagent (dithiothreitol) and a proteolytic enzyme (papain or ficin). This reagent dissociates IgG and complement from red blood cells, allowing for phenotyping, enhanced adsorption, or denaturing of multiple blood group system antigens to aid in completing complex antibody workups. ZZAP treatment destroys all antigens in the Kell, Landsteiner- Wiener, Cartwright, Dombrock, and Knops blood group systems as well as antigens destroyed by proteases (e.g., M, N, S, Fya, and Fyb).

摘要

ZZAP是一种巯基试剂（二硫苏糖醇）和一种蛋白水解酶（木瓜蛋白酶或无花果蛋白酶）的混合物。该试剂可使免疫球蛋白G和补体与红细胞解离，从而有助于血型鉴定、增强吸附或使多种血型系统抗原变性，以辅助完成复杂的抗体检测。ZZAP处理会破坏凯尔血型系统、兰斯泰纳-维纳血型系统、卡特赖特血型系统、多姆布罗克血型系统和诺普斯血型系统中的所有抗原，以及被蛋白酶破坏的抗原（如M、N、S、Fya和Fyb）。