Hospital General de Niños Pedro de Elizalde, Ciudad Autónoma de Buenos Aires, Argentina.
Arch Argent Pediatr. 2022 Oct;120(5):336-339. doi: 10.5546/aap.2022.eng.336. Epub 2022 Aug 19.
Stopping the spread of coronavirus disease 2019 (COVID-19) is critical and can be achieved through rapid and effective detection techniques. Our objective was to compare the diagnostic accuracy of rapid antigen tests (RAgT) and reverse transcriptionquantitative polymerase chain reaction (RT-qPCR) and to describe amplification cycle thresholds (Cts). Participants were children aged 1 month to 11 years with symptoms for less than 7 days, who did not have a detectable result in the past 90 days, and were immunocompetent. A total of 1855 patients were included; the prevalence of COVID-19 was 4.7%. For the RAgT, overall sensitivity was 60.2% and specificity, 99.8%; in children older than 5 years, values were 69.8% and 99.8%, respectively. Ct values for discordant samples were higher. To conclude, the diagnostic accuracy indicated that the specificity of RAgT is similar to that of RT-qPCR, but its sensitivity is notably lower, especially in children younger than 5 years.
阻止 2019 年冠状病毒病（COVID-19）的传播至关重要，可以通过快速有效的检测技术实现。我们的目的是比较快速抗原检测（RAgT）和逆转录定量聚合酶链反应（RT-qPCR）的诊断准确性，并描述扩增循环阈值（Cts）。参与者为 1 个月至 11 岁有症状且不足 7 天、在过去 90 天内无检测结果、免疫功能正常的儿童。共纳入 1855 例患者；COVID-19 的患病率为 4.7%。对于 RAgT，总敏感性为 60.2%，特异性为 99.8%；5 岁以上儿童的敏感性分别为 69.8%和 99.8%。不一致样本的 Ct 值更高。总之，诊断准确性表明，RAgT 的特异性与 RT-qPCR 相似，但敏感性明显较低，尤其是 5 岁以下儿童。
