Kawamata S
Anat Rec. 1987 Jun;218(2):223-8. doi: 10.1002/ar.1092180218.
Tree frogs were loaded with strontium chloride (SrCl2). The incorporation of strontium metal into the calcium carbonate (CaCO3) crystals located both in the inner ear and in the endolymphatic sac was studied by x-ray microanalysis (XMA) and scanning electron microscopy (SEM). In the inner ear, strontium was not recognized except for traces in a few crystals. When observed by SEM, these crystals had a faceted body and two pointed ends with rather smooth surfaces. However, in the endolymphatic sac, which greatly expands into the spinal canal, strontium was clearly present at every surface of all crystals. Careful examinations by point and line XMA revealed that strontium x-ray counts were highest at the pointed ends and decreased sharply and then gradually toward the equator of the crystals. SEM observations revealed that the crystals in the endolymphatic sac always had rough and irregular surfaces regardless of their shapes and sizes. Calcium was always found in crystals of both organs. Except for calcium and strontium, other elements including sodium and heavier elements were negligible in XMA. These findings suggest that strontium is incorporated into the crystals only in the endolymphatic sac, and the rough-surfaced covering of these crystals reflects newly deposited strontium salt. It seems to indicate that these crystals grow predominantly by accretion.
给树蛙注射氯化锶（SrCl₂）。通过X射线微分析（XMA）和扫描电子显微镜（SEM）研究锶金属在位于内耳和内淋巴囊的碳酸钙（CaCO₃）晶体中的掺入情况。在内耳中，除了少数晶体中有微量痕迹外，未检测到锶。用SEM观察时，这些晶体有一个多面的主体和两个尖端，表面相当光滑。然而，在内淋巴囊（它极大地延伸到椎管内）中，所有晶体的每个表面都明显存在锶。通过点和线XMA仔细检查发现，锶的X射线计数在晶体的尖端最高，然后急剧下降，接着朝着晶体的赤道逐渐降低。SEM观察表明，内淋巴囊中的晶体无论其形状和大小如何，表面总是粗糙且不规则。在这两个器官的晶体中都始终能发现钙。除了钙和锶，XMA中包括钠和更重元素在内的其他元素可忽略不计。这些发现表明，锶仅在内淋巴囊的晶体中掺入，并且这些晶体表面粗糙的覆盖层反映了新沉积的锶盐。这似乎表明这些晶体主要通过堆积生长。
