Kawamata S
Department of Anatomy, Toyama Medical and Pharmaceutical University, Japan.
Arch Histol Cytol. 1990 Oct;53(4):397-404. doi: 10.1679/aohc.53.397.
The growth rate of calcium carbonate (CaCO3) crystals in the endolymphatic sac was modulated, and morphological changes in this organ were observed by light and electron microscopy. When calcium chloride (CaCl2) was given to the three frog for a short period (3 days to 2 weeks), CaCO3 crystal production was accelerated. Epithelial cells enlarged, their rough endoplasmic reticulum (rER) and Golgi apparatus developed, and dense material increased around CaCO3 crystals and/or in the endolymphatic lumen. In addition, multiluminal endolymphatic chambers appeared in some frogs. On the other hand, as the CaCl2 loading period lengthened and CaCO3 crystal formation decreased or stopped, the epithelial cells became flat and extended with scanty cytoplasm, and the rER and Golgi apparatus decreased in number and size. Furthermore, the amount of dense material around CaCO3 crystals and in the lumen decreased. These findings suggest that the rER, Golgi apparatus and dense material have key roles in the production of CaCO3 crystals.
碳酸钙（CaCO₃）晶体在内淋巴囊中的生长速率受到调节，并通过光学显微镜和电子显微镜观察该器官的形态变化。当给三只青蛙短期（3天至2周）注射氯化钙（CaCl₂）时，CaCO₃晶体的产生加速。上皮细胞增大，其粗面内质网（rER）和高尔基体发育，并且CaCO₃晶体周围和/或内淋巴腔中的致密物质增加。此外，一些青蛙出现了多腔的内淋巴腔。另一方面，随着CaCl₂加载期延长且CaCO₃晶体形成减少或停止，上皮细胞变得扁平并伸展，细胞质稀少，rER和高尔基体的数量和大小减少。此外，CaCO₃晶体周围和腔内的致密物质数量减少。这些发现表明，rER、高尔基体和致密物质在CaCO₃晶体的产生中起关键作用。
