Department of Dermatology, Copenhagen University Hospital - Bispebjerg, Copenhagen, Denmark;
Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark.
Anticancer Res. 2022 Oct;42(10):5083-5090. doi: 10.21873/anticanres.16017.
BACKGROUND/AIM: The effect of vitamin D on skin carcinogenesis is unclear. Vitamin D derivatives may protect against ultraviolet radiation (UVR)-induced DNA damage, immune suppression, and skin carcinogenesis. However, some epidemiological studies have reported an increased incidence of skin cancer associated with high serum vitamin D levels. We investigated the effect of vitamin D supplementation on serum, skin, and tumor vitamin D levels and on skin cancer development in hairless immunocompetent mice.
Female C3.Cg-Hr/TifBomTac immunocompetent mice (n=125) were randomly separated into five groups. Two groups received a high vitamin D diet (4.5 μg/day/mouse). One group received a medium vitamin D diet (2.3 μg/day/mouse). Two groups received a standard diet (0.045 μg/day/mouse). Three standard erythema doses of UVR were given three times per week to three groups.
Animals on a high vitamin D3 diet had ~150-fold higher serum vitamin D levels (p=0.00016) and 3-fold higher serum 25-hydroxyvitamin D [25(OH)D] levels (p=0.00016) than those on a standard diet. For mice on the medium vitamin D diet, serum vitamin D and 25(OH)D levels were 18-fold and 2.3-fold higher than for the standard diet, respectively (p=0.00016). All UVR-exposed mice developed tumors. Vitamin D levels were lower in the tumor than the skin (p<0.0001). High and medium supplementation with vitamin D did not affect tumor development (p>0.05).
In mice, vitamin D levels in the serum, skin, and tumors were augmented by supplementation, but this did not affect the development of UVR-induced skin tumors.
背景/目的：维生素 D 对皮肤致癌作用的影响尚不清楚。维生素 D 衍生物可能具有预防紫外线（UVR）诱导的 DNA 损伤、免疫抑制和皮肤致癌作用。然而，一些流行病学研究报告称，高血清维生素 D 水平与皮肤癌发病率增加有关。我们研究了维生素 D 补充对无毛免疫功能正常的小鼠血清、皮肤和肿瘤维生素 D 水平以及皮肤癌发展的影响。
将 125 只雌性 C3.Cg-Hr/TifBomTac 免疫功能正常的小鼠随机分为五组。两组给予高维生素 D 饮食（4.5 μg/天/只）。一组给予中维生素 D 饮食（2.3 μg/天/只）。两组给予标准饮食（0.045 μg/天/只）。三组标准红斑剂量的 UVR 每周给予三组三次。
高维生素 D3 饮食组的动物血清维生素 D 水平高 150 倍（p=0.00016），血清 25-羟维生素 D [25（OH）D]水平高 3 倍（p=0.00016）高于标准饮食组。对于中维生素 D 饮食组，血清维生素 D 和 25（OH）D 水平分别比标准饮食组高 18 倍和 2.3 倍（p=0.00016）。所有 UVR 暴露的小鼠均发生肿瘤。肿瘤中的维生素 D 水平低于皮肤（p<0.0001）。高和中剂量的维生素 D 补充均未影响肿瘤的发展（p>0.05）。
在小鼠中，血清、皮肤和肿瘤中的维生素 D 水平通过补充得到增强，但这并未影响 UVR 诱导的皮肤肿瘤的发展。
