Department of Nanoscience and Engineering, Inje University, Gimhae, 50834, Republic of Korea.
College of Biology and the Environment, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu, 210037, China; National Positioning Observation Station of Hung-tse Lake Wetland Ecosystem in Jiangsu Province, Hongze, Jiangsu, 223100, China.
Anal Chim Acta. 2022 Oct 16;1230:340403. doi: 10.1016/j.aca.2022.340403. Epub 2022 Sep 16.
In this study, we developed a novel galloyl group-functionalized polydiacetylene (Galloyl-PDA) sensor for colorimetric and fluorescent detection of Pb. Among three types of Galloyl-PDA vesicles prepared by changing the ratio of newly synthesized galloyl group-conjugated 10,12-pentacosadiynoic acid (Galloyl-PCDA) and matrix 10,12-tricosadinoic acid (TCDA), the blue Galloyl-PDA vesicles with 1:9 molar ratio of Galloyl-PCDA:TCDA showed the most dramatic color transitions to red with colorimetric response (CR) value of 46.66 ± 1.373% within 5 min upon addition of 50 μM Pb. However, they didn't exhibit any color change upon interaction with other heavy metals. Since the terminal galloyl moieties of the Galloyl-PDA vesicles could form coordination bonds with Pb, the Galloyl-PDA vesicles were stressed and showed obvious blue-to-red chromatic transitions. Besides, because the Galloyl-PDA vesicles exhibited nonfluorescent-to-fluorescent transitions, a linear response in colorimetric and fluorescent signals was observed in the range of 0-10 μM and 0.025-1 μM, respectively. From the colorimetric and fluorescent results, the limit of detection (LOD) was determined to be 1.329 μM and 0.068 μM, which is 8-fold and 12-fold better sensitivity than those of previously reported methods, respectively. Furthermore, the capability of our PDA sensor for detection of Pb in tap water, river water, and human serum was validated with excellent precision and recovery rates of 97.14-100.0%, 99.05-103.3%, and 100.7-106.7%, respectively. As our PDA dual-signal sensor for Pb is rapid, sensitive, specific, and detectable by the naked eye, this approach holds great promise for application in point-of-care testing (POCT).
在这项研究中，我们开发了一种新型的没食子酰基功能化聚二乙炔（Galloyl-PDA）传感器，用于比色和荧光检测 Pb。在所制备的三种不同类型的 Galloyl-PDA 囊泡中，通过改变新合成的没食子酰基缀合的 10,12-二十五碳二炔酸（Galloyl-PCDA）与基质 10,12-二十三碳二炔酸（TCDA）的比例，当 Galloyl-PCDA:TCDA 的摩尔比为 1:9 时，所得的蓝色 Galloyl-PDA 囊泡在 5 分钟内对 50 μM Pb 的比色响应（CR）值为 46.66 ± 1.373%，表现出最显著的颜色转变为红色。然而，它们与其他重金属相互作用时没有任何颜色变化。由于 Galloyl-PDA 囊泡的末端没食子酰基部分可以与 Pb 形成配位键，因此 Galloyl-PDA 囊泡受到压力并表现出明显的蓝到红的颜色转变。此外，由于 Galloyl-PDA 囊泡表现出非荧光到荧光的转变，因此在 0-10 μM 和 0.025-1 μM 的范围内观察到比色和荧光信号的线性响应。根据比色和荧光结果，检测限（LOD）确定为 1.329 μM 和 0.068 μM，这分别比之前报道的方法提高了 8 倍和 12 倍的灵敏度。此外，我们的 PDA 传感器用于检测自来水中、河水中和人血清中的 Pb 的能力得到了验证，其精密度和回收率分别为 97.14-100.0%、99.05-103.3%和 100.7-106.7%。由于我们的 PDA 双信号传感器对 Pb 的检测具有快速、灵敏、特异性和可通过肉眼观察，因此该方法有望在即时检测（POCT）中得到应用。
