Cystic echinococcosis is a zoonotic disease caused by the parasite sensu lato (s.l.), which is worldwide distributed and causes long-lasting infections in animals and humans. The existing treatment is limited to the use of benzimidazoles, mainly albendazole (ABZ). However, it has unwanted side effects and its efficacy is about 50%. The Asteraceae family includes plants that have therapeutic applications (medicinal species) and has an important role in new drug development. The species belonging to a different genus of this family show a wide range of anti-inflammatory, antimicrobial, antioxidant, hepatoprotective, and antiparasitic activities, among others. The aim of the present study was to evaluate the efficacy of extracts of four Asteraceae species against protoscoleces of sensu stricto (s.s.). On the other hand, the extract was assessed on the murine cyst of (s.s.) and the efficacy of extract was investigated in a murine model of CE. , , and extracts at 100 g/mL caused a decrease in protoscoleces viability; however, extract produced the greatest protoscolicidal effect. After 20 days of treatment with the highest concentration (100 g/mL) of extract, protoscoleces viability decreased to 0%. The tegumental changes observed by scanning electron microscopy were consistent with the reduction in vitality. The collapse of the germinal layer was registered in 60 ± 5.8% and 83.3 ± 12.0% of cysts treated during 4 days with 50 and 100 g/ml, respectively. The half maximal effective concentration (EC50) value of the extract against (s.s.) cysts was 47.86 g/mL (96 h). The dosage of infected animals with the 50 mg kg dose of extract resulted in a significant reduction in cyst weight in comparison with the control group. In conclusion, extract was demonstrated to exert a marked effect, both in vitro and in the murine model.