Kaya Yasin, Aydın Zübeyde Uğurlu, Cai Xu, Wang Xiaowu, Dönmez Ali A
Molecular Plant Systematic Laboratory (MOBIS), Department of Biology, Faculty of Science, Hacettepe University, Ankara 06800, Turkey.
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
AoB Plants. 2022 Sep 10;14(5):plac035. doi: 10.1093/aobpla/plac035. eCollection 2022 Oct.
complex is divided into two subspecies, subsp, subsp. and a distinct species, , based on molecular phylogeny. We generated a draft assembly of subsp. and using paired-end shotgun sequencing. This is the first attempt at genome characterization for the genus. In the presented study, ~165 and ~157 Mbp of the genomes of subsp. and were assembled, respectively, and a total of 32 425 and 31 372 gene models were predicted in the genomes of the target taxa, respectively. We corroborated the phylogenomic affinity of taxa with some core Brassicaceae species (Clades A and B) including . The orthology-based tree suggested that species differentiated from 1.3-2.0 mya (million years ago). The genome-wide syntenic comparison of two taxa revealed that subsp. (46 %) and (45 %) have an almost identical syntenic gene pair ratio. These novel genome assemblies are the first steps towards the chromosome-level assembly of and understanding the genome diversity within the genus.
根据分子系统发育，复合体被分为两个亚种，即亚种 和亚种 ，以及一个独特的物种， 。我们使用双末端鸟枪法测序生成了亚种 和 的草图组装。这是该属基因组特征描述的首次尝试。在本研究中，分别组装了亚种 和 的约165 Mbp和约157 Mbp的基因组，并且在目标分类群的基因组中分别预测了总共32425个和31372个基因模型。我们证实了该分类群与一些十字花科核心物种（A和B分支）包括 的系统发育亲缘关系。基于直系同源的树状图表明， 物种在130 - 200万年前与 分化。两个 分类群的全基因组共线性比较显示，亚种 （46%）和 （45%）具有几乎相同的共线性基因对比率。这些新的基因组组装是迈向 染色体水平组装以及理解该属内基因组多样性的第一步。
