Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Department of Developmental Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Chembiochem. 2022 Dec 5;23(23):e202200297. doi: 10.1002/cbic.202200297. Epub 2022 Nov 9.
A wide array of optogenetic tools are available that allow for precise spatiotemporal control over cellular processes. These tools are particularly important to zebrafish researchers who take advantage of the embryo's transparency. However, photocleavable optogenetic proteins have not been utilized in zebrafish. We demonstrate successful optical control of protein cleavage in embryos using PhoCl, a photocleavable fluorescent protein. This optogenetic tool offers temporal and spatial control over protein cleavage events, which we demonstrate in light-triggered protein translocation and light-triggered apoptosis.
有各种各样的光遗传学工具可供使用，这些工具可以精确地控制细胞过程的时空。这些工具对利用胚胎透明性的斑马鱼研究人员特别重要。然而，光可裂解的光遗传学蛋白尚未在斑马鱼中得到应用。我们使用光可裂解的荧光蛋白 PhoCl，证明了在胚胎中对蛋白质切割的成功光学控制。这种光遗传学工具提供了对蛋白质切割事件的时间和空间控制，我们在光触发的蛋白质转位和光触发的细胞凋亡中证明了这一点。
