Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Laboratório de Engenharia e Exploração de Petróleo (LENEP), Rodovia Amaral Peixoto, Km 163, Avenida Brennand, Imboassica, 27925-535 Macaé, RJ, Brazil.
Universidade Federal do Rio de Janeiro (UFRJ), Instituto de Química, Avenida Athos da Silveira Ramos, 149, Ilha do Fundão, 21941-909 Rio de Janeiro, RJ, Brazil.
An Acad Bras Cienc. 2022 Oct 3;94(suppl 3):e20211433. doi: 10.1590/0001-3765202220211433. eCollection 2022.
Biosurfactants and waterflooding have been widely reported thus far for enhancing oil production. Nevertheless, there is a lack of literature to explore enhanced oil recovered methods effects on its chemical composition. The aim of this work is to investigate the effects of a biosurfactant produced by Bacillus safensis and brine injection on the recovered petroleum composition, and their implications for geochemical interpretation. Original and oils recovered from displacement tests were analyzed by gas chromatography and ultra-high-resolution mass spectrometry, emphasizing saturated and aromatic biomarkers and basic and acidic polar compounds. Geochemical parameters based on some saturated compounds were subtly affected by the recovery methods, showing their reliable applicability in geochemical studies. Contrarily, parameters based on some aromatic compounds were more affected by biosurfactant flooding, mostly the low molecular weight compounds. Thus, these aromatic parameters should be applied with caution after such methods. The distribution of basic and acidic polar compounds can also be modified affecting the geochemical interpretation. In the case of the basic ones, the biosurfactant greatly influenced the N class species with favorable loss of lower aromaticity compounds. In addition to water solubilization, the compositional changes described in this study can be related to fractionation due to adsorption on reservoir rocks.
迄今为止，生物表面活性剂和注水已被广泛报道用于提高石油产量。然而，目前缺乏文献探讨强化采油方法对其化学成分的影响。本工作旨在研究由芽孢杆菌 safensis 产生的生物表面活性剂和盐水注入对回收石油组成的影响，以及它们对地球化学解释的意义。通过气相色谱和超高分辨率质谱分析了原始油和驱替试验回收的油，重点分析了饱和和芳香生物标志物以及碱性和酸性极性化合物。一些基于饱和化合物的地球化学参数受到回收方法的细微影响，表明它们在地球化学研究中具有可靠的适用性。相反，基于一些芳香族化合物的参数受生物表面活性剂驱油的影响更大，主要是低分子量化合物。因此，在使用这些方法后，这些芳香族参数的应用应谨慎。碱性和酸性极性化合物的分布也可能发生变化，从而影响地球化学解释。就碱性化合物而言，生物表面活性剂极大地影响了 N 类物质，有利于降低低芳香度化合物的损失。除了水增溶作用外，本研究中描述的组成变化还可能与由于在储层岩石上的吸附而导致的分馏有关。
