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体外实验中，长叶松树皮提取物及其馏分对巴西利什曼原虫的活性。

In vitro activity of Schinus terebinthifolius extract and fractions against Sporothrix brasiliensis.

机构信息

Fundação Oswaldo Cruz-Fiocruz, Instituto Nacional de Infectologia Evandro Chagas, Laboratório de Micologia, Rio de Janeiro, RJ, Brasil.

Fundação Oswaldo Cruz-Fiocruz, Instituto de Tecnologia em Fármacos, Laboratório de Química de Produtos Naturais, TecBio/LDFito, Far-Manguinhos, Rio de Janeiro, RJ, Brasil.

出版信息

Mem Inst Oswaldo Cruz. 2022 Sep 30;117:e220063. doi: 10.1590/0074-02760220063. eCollection 2022.

DOI:10.1590/0074-02760220063
PMID:36197404
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9524759/
Abstract

BACKGROUND

Sporothrix brasiliensis is the causative agent of zoonotic cases of sporotrichosis in Brazil and is associated with atypical and severe presentations in cats, dogs, and humans. Sporotrichosis treatment is usually time- and cost-consuming, sometimes with poor response and host toxicity. Schinus terebinthifolius has proven efficacy against bacteria and fungi of clinical interest.

OBJECTIVE

To determine the in vitro activity of S. terebinthifolius against S. brasiliensis.

METHODS

Five S. brasiliensis isolates and three reference strains were subjected to a hydroethanol extract derived from the leaves of S. terebinthifolius and its fractions. The minimal inhibitory concentration (MIC) was determined using the broth microdilution method according to the M38-A2 CLSI guidelines. Also, the fungicidal/fungistatic activity of the extract and fractions was studied.

FINDINGS

The crude extract of S. terebinthifolius inhibited the growth of S. brasiliensis (MIC: 0.5-1.0 µg/mL), while the partitioned extracts dichloromethane, ethyl acetate, and butanol demonstrated growth inhibition at 8 µg/mL due to a fungistatic activity.

MAIN CONCLUSIONS

Due to its in vitro efficacy against S. brasiliensis and its known pharmacological safety, S. terebinthifolius is a candidate to be tested using in vivo models of sporotrichosis.

摘要

背景

巴西生孢梭菌是巴西动物源性孢子丝菌病的病原体，与猫、狗和人类的非典型和严重表现有关。孢子丝菌病的治疗通常既费时又费钱，有时反应不佳且宿主毒性大。瓶刷树对临床相关的细菌和真菌具有良好的疗效。

目的

测定瓶刷树叶的水-乙醇提取物及其各馏分对巴西生孢梭菌的体外活性。

方法

对 5 株巴西生孢梭菌分离株和 3 株参考菌株进行了瓶刷树叶水-乙醇提取物及其各馏分的处理。采用肉汤微量稀释法，根据 CLSI M38-A2 指南测定最小抑菌浓度（MIC）。同时，还研究了提取物及其馏分的杀菌/抑菌活性。

发现

瓶刷树的粗提取物抑制了巴西生孢梭菌的生长（MIC：0.5-1.0μg/ml），而二氯甲烷、乙酸乙酯和正丁醇等分馏提取物由于具有抑菌活性，在 8μg/ml 时表现出抑制生长的作用。

主要结论

鉴于其对巴西生孢梭菌的体外疗效以及已知的药理学安全性，瓶刷树可作为孢子丝菌病体内模型的候选药物进行测试。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1404/9524759/cfcdbef1aab1/1678-8060-mioc-117-e220063-gf3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1404/9524759/f7124a3538f8/1678-8060-mioc-117-e220063-gf1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1404/9524759/5be6a0a3d156/1678-8060-mioc-117-e220063-gf2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1404/9524759/cfcdbef1aab1/1678-8060-mioc-117-e220063-gf3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1404/9524759/f7124a3538f8/1678-8060-mioc-117-e220063-gf1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1404/9524759/5be6a0a3d156/1678-8060-mioc-117-e220063-gf2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1404/9524759/cfcdbef1aab1/1678-8060-mioc-117-e220063-gf3.jpg

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