Clinical Laboratory, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, China.
Department of Clinical Laboratory Medicine, Xijing Hospital, Fourth Military Medical University (Air Force Medical University), 127 Changle West Road, 710032, Xi'an, Shaanxi, China.
BMC Nephrol. 2022 Oct 5;23(1):328. doi: 10.1186/s12882-022-02953-x.
This study is aimed to evaluate if automated urine sediment analysis UN2000 can be used to screen lupus nephritis.
UN2000 was used to examine 160 urine samples from patients with systemic lupus erythematosus and 124 urine samples from Lupus nephritis. The result of protein/creatinine ratio(P/C) and renal tubular epithelial cells (RTEC) were evaluated. With biochemical analysis and microscopic examination as the gold standard, the Kappa consistency test was used to analyze the accuracy of P/C and RTEC. Analysis was to evaluate the accuracy of P/C single item or RTEC single item and both screening lupusnephritis.
The consistency of P/C and the gold standard, and that of RTEC and the gold standard are respectively strong and good (0.858 vs. 0.673). The specificity, positive predictive value, and coincidence were the highest when P/C ≥ 2 + was set as the only screening standard for lupus nephritis. When the standard was selected between P/C ≥ 2 + or RTEC > 2.8 cells/µl, the sensitivity and negative predictive value were the highest.
UN 2000 can be used to screen lupus nephritis by detecting P/C and RTEC.
本研究旨在评估 UN2000 自动尿沉渣分析是否可用于筛查狼疮肾炎。
使用 UN2000 检测 160 例系统性红斑狼疮患者和 124 例狼疮肾炎患者的 160 例尿液样本。评估蛋白/肌酐比值（P/C）和肾小管上皮细胞（RTEC）的结果。以生化分析和显微镜检查为金标准，采用 Kappa 一致性检验分析 P/C 和 RTEC 的准确性。分析 P/C 单项或 RTEC 单项及两者联合筛查狼疮肾炎的准确性。
P/C 与金标准的一致性以及 RTEC 与金标准的一致性分别为强和良好（0.858 比 0.673）。当将 P/C≥2+作为狼疮肾炎唯一的筛查标准时，其特异性、阳性预测值和符合率最高。当 P/C≥2+或 RTEC>2.8 细胞/µl 之间作为标准时，其敏感性和阴性预测值最高。
UN2000 可通过检测 P/C 和 RTEC 用于筛查狼疮肾炎。
