大豆中诱导型硝酸还原酶同工型的转录调控。

Transcriptional control of the inducible nitrate reductase isoform from soybeans.

作者信息

Smarrelli J, Malone M J, Watters M T, Curtis L T

出版信息

Biochem Biophys Res Commun. 1987 Aug 14;146(3):1160-5. doi: 10.1016/0006-291x(87)90769-8.

DOI:10.1016/0006-291x(87)90769-8

Abstract

The activity of the pH 7.5 NADH-linked nitrate reductase isoform from soybean seedlings is termed inducible. Activity is present only in the leaves of seedlings which have been supplied nitrate. A cDNA clone that encoded part of the mRNA for squash nitrate reductase hybridized specifically with mRNA for this inducible nitrate reductase isoform. Nitrate induction resulted in an increase in the steady-state levels of mRNA for this isoform after 24 hours, while the addition of glutamine to the nitrate diminished steady-steady state levels of this mRNA.

摘要

来自大豆幼苗的pH 7.5的NADH连接的硝酸还原酶同工型的活性被称为可诱导的。该活性仅存在于已供应硝酸盐的幼苗叶片中。一个编码南瓜硝酸还原酶部分mRNA的cDNA克隆与这种可诱导的硝酸还原酶同工型的mRNA特异性杂交。硝酸盐诱导导致24小时后该同工型mRNA的稳态水平增加，而向硝酸盐中添加谷氨酰胺则降低了该mRNA的稳态水平。

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引用本文的文献

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Identification of cDNA clones corresponding to two inducible nitrate reductase genes in soybean: analysis in wild-type and nr1 mutant.大豆中两个诱导型硝酸还原酶基因对应cDNA克隆的鉴定：野生型和nr1突变体分析

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Plant Mol Biol. 1995 Jan;27(1):129-36. doi: 10.1007/BF00019184.

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