Baghban Hossein Navay, Hasanzadeh Mohammad, Liu Yuqian, Seidi Farzad
Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources and International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University Nanjing 210037 China
Food and Drug Safety Research Center, Tabriz University of Medical Sciences Tabriz Iran.
RSC Adv. 2022 Sep 8;12(39):25675-25686. doi: 10.1039/d2ra04793d. eCollection 2022 Sep 5.
In recent years the use of ractopamine (RAC), originally synthesized for the treatment of respiratory diseases, is on the rise as a dietary supplement in animals. The excessive use of RAC has some adverse effects on human health. Hence, the demand for simple, easy-to-use, and expendable devices for RAC recognition, even in remote areas, is felt more than ever before. This need prompted us to devise a straightforward colorimetric system for RAC recognition based on the etching effect of RAC on AgNPrs. This nanoprobe is a very advanced materials with great optical properties and stability, which could be used unprecedentedly without any combination or reagents for RAC recognition. Considering the needs and advantages, a simple colorimetric chemosensor for the quantification of RAC was designed and applied to a chicken sample. The designed chemosensor was integrated with an optimized microfluidic paper-based colorimetric device (μPCD), creating a suitable tool for the determination of RAC based on a time/color pattern. The analytical metrics for this simple colorimetric chemosensing regime comprise a best colorimetric LLOQ of 100 μM in solution with 10 μM of μPCD, a spectroscopic LLOQ of 10 nM, and a broad linearity range of 0.1-10 000 μM, which are outstanding compared with other colorimetric techniques. The main remarkable features of this study include the first utilization of AgNPrs with high stability and excellent optical properties without any reagent as an optical sensing probe and optimized μPCD toward RAC recognition and the innovative time/color semi-analytical recognition method. Moreover, the prepared portable μPCD modified with AgNPrs could be a prized candidate for commercialization due to the benefits of the low-cost materials used, like paper and paraffin, and the simple instructions for μPCD preparation. This report could be a pioneering work, inspiring simple and effective on-site semi-analytical recognition devices for harmful substances or illegal drugs, which simply consist of a piece of lightweight paper and one drop of the required reagent.
近年来，原本用于治疗呼吸系统疾病的莱克多巴胺（RAC）作为动物膳食补充剂的使用量正在上升。RAC的过度使用对人体健康有一些不利影响。因此，人们比以往任何时候都更强烈地感受到对简单、易用且一次性的RAC识别设备的需求，即使在偏远地区也是如此。这种需求促使我们设计了一种基于RAC对银纳米粒子（AgNPrs）蚀刻效应的简单比色系统用于RAC识别。这种纳米探针是一种具有出色光学性质和稳定性的非常先进的材料，前所未有的是它无需任何组合或试剂即可用于RAC识别。考虑到需求和优势，设计了一种用于定量RAC的简单比色化学传感器，并将其应用于鸡肉样本。所设计的化学传感器与优化的基于微流控纸的比色装置（μPCD）集成在一起，创建了一种基于时间/颜色模式测定RAC的合适工具。这种简单比色化学传感方法的分析指标包括：在含有10 μM μPCD的溶液中比色最低定量限（LLOQ）为100 μM，光谱LLOQ为10 nM，线性范围宽达0.1 - 10000 μM，与其他比色技术相比非常出色。本研究的主要显著特点包括首次将具有高稳定性和出色光学性质且无需任何试剂的AgNPrs用作光学传感探针，并针对RAC识别对μPCD进行了优化，以及创新的时间/颜色半分析识别方法。此外，由于使用了低成本材料（如纸张和石蜡）以及μPCD制备说明简单，制备的用AgNPrs修饰的便携式μPCD可能是商业化的理想候选产品。本报告可能是一项开创性工作，激发了用于有害物质或非法药物的简单有效的现场半分析识别设备的开发，这些设备仅由一张轻质纸和一滴所需试剂组成。
