Farshchi Fatemeh, Saadati Arezoo, Hasanzadeh Mohammad, Seidi Farzad
Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University Nanjing 210037 China
Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences Tabriz Iran
RSC Adv. 2021 Aug 10;11(44):27298-27308. doi: 10.1039/d1ra04764g. eCollection 2021 Aug 9.
Uric acid (UA) is the end product of purine metabolism. Uric acid is usually excreted in the urine, but its abnormal increase and toxic amount can lead to diseases such as gout, hyperuricemia, Lesch-Nyhan syndrome, and cardiovascular disease. On the other hand, UA reduction can lead to neurodegenerative diseases such as sarcoma, glioblastoma, Hodgkin, and Therefore, rapid identification of UA is of great importance. In this work, a simple, portable, inexpensive, and fast microfluidic paper-based colorimetric sensor based on the color change in the presence of UA by using AuNPs was developed. The results can be easily identified with naked eye and further confirmed by UV-vis spectrophotometry. In this method, iron pattern and fiberglass paper were used to construct diagnostic areas and hydrophilic microfluidic channels. We greatly reduced the preparation time of this pattern using a magnet (about three minutes). In this work, four types of nanoparticles with different lower limit of quantification (LLOQ) were used. Linear range of 10 to 10 M and LLOQ of 10 M were obtained for the determination of uric acid using AuNPs-CysA as optical probe. Also, by AuNPs as optical probe a linear range of 10 to 10 M and the obtained LLOQ was 10 M. Finally, by AuNFs as optical probe linear range from 10 to 10 M and 5 × 10 to 10 M along with LLOQ of 10 and 5 × 10 M, respectively. The designed system successfully studied in human urine samples.
尿酸（UA）是嘌呤代谢的终产物。尿酸通常通过尿液排出，但尿酸异常增加及其毒性量会导致痛风、高尿酸血症、莱施-奈恩综合征和心血管疾病等疾病。另一方面，尿酸降低会导致肉瘤、胶质母细胞瘤、霍奇金淋巴瘤等神经退行性疾病。因此，快速识别尿酸非常重要。在这项工作中，开发了一种基于金纳米颗粒（AuNPs）在尿酸存在下颜色变化的简单、便携、廉价且快速的微流控纸基比色传感器。结果可用肉眼轻松识别，并通过紫外可见分光光度法进一步确认。在该方法中，使用铁模板和玻璃纤维纸构建诊断区域和亲水微流控通道。我们使用磁铁大大缩短了这种模板的制备时间（约三分钟）。在这项工作中，使用了四种具有不同定量下限（LLOQ）的纳米颗粒。以AuNPs-CysA作为光学探针测定尿酸时，线性范围为10至10 M，LLOQ为10 M。同样，以AuNPs作为光学探针时，线性范围为10至10 M，获得的LLOQ为10 M。最后，以AuNFs作为光学探针时，线性范围分别为10至10 M和5×10至10 M，LLOQ分别为10和5×10 M。所设计的系统在人体尿液样本中成功进行了研究。
