Song Na-Na, Qian Guan-Yu, Zheng Hai-Lin, Zhou Xiao-Wei, Mei Huan, Li Dong-Mei, Li Xiao-Fang, Liu Wei-Da
Department of Mycology, Hospital for Skin Diseases (Institute of Dermatology), Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu 210042, China.
Department of Dermatology, Xuzhou Tumor Hospital, Xuzhou, Jiangsu 221000, China.
Int J Dermatol Venereol. 2022 Sep;5(3):132-139. doi: 10.1097/JD9.0000000000000223. Epub 2022 Jan 19.
By assessing and comparing the phenotypic changes on the stepwise acquisition of fluconazole resistant isolates, we could find and describe the relationship between drug resistance and biofilm formation ability in a series of clonal strains.
We performed antifungal susceptibility of five drugs (fluconazole, itraconazole, voriconazole, caspofungin and amphotericin B) to further verify the antifungal activity of the six isolates . Then we combined hyphal formation assay, cell surface hydrophobicity test positively related to adherence ability, and biofilm assays to observe and compare the phenotypic characteristics of our six clonal strains.
Biofilm capability is enhanced for four drug- intermediate strains, whereas the initial susceptible strain and the final resistant strain are both poor in adherence, hyphal growth and biofilm formation.
It was suggested that the biofilm formation ability were not absolutely related to the degree of fluconazole resistance.
通过评估和比较逐步获得氟康唑耐药菌株过程中的表型变化，我们能够发现并描述一系列克隆菌株中耐药性与生物膜形成能力之间的关系。
我们对五种药物（氟康唑、伊曲康唑、伏立康唑、卡泊芬净和两性霉素B）进行了抗真菌药敏试验，以进一步验证六种菌株的抗真菌活性。然后，我们结合菌丝形成试验、与黏附能力呈正相关的细胞表面疏水性测试以及生物膜试验，来观察和比较我们六种克隆菌株的表型特征。
四种药物中介菌株的生物膜形成能力增强，而初始敏感菌株和最终耐药菌株在黏附、菌丝生长和生物膜形成方面均较差。
提示生物膜形成能力与氟康唑耐药程度并非绝对相关。
