Biofilm Alterations on the Stepwise Acquisition of Fluconazole-resistant Isolates.

UNLABELLED

By assessing and comparing the phenotypic changes on the stepwise acquisition of fluconazole resistant isolates, we could find and describe the relationship between drug resistance and biofilm formation ability in a series of clonal strains.

METHODS

We performed antifungal susceptibility of five drugs (fluconazole, itraconazole, voriconazole, caspofungin and amphotericin B) to further verify the antifungal activity of the six isolates . Then we combined hyphal formation assay, cell surface hydrophobicity test positively related to adherence ability, and biofilm assays to observe and compare the phenotypic characteristics of our six clonal strains.

RESULTS

Biofilm capability is enhanced for four drug- intermediate strains, whereas the initial susceptible strain and the final resistant strain are both poor in adherence, hyphal growth and biofilm formation.

CONCLUSIONS

It was suggested that the biofilm formation ability were not absolutely related to the degree of fluconazole resistance.