Charles River Laboratories, Mattawan, Michigan, USA.
Inotiv, Boulder, Colorado, USA.
Toxicol Pathol. 2022 Dec;50(8):904-909. doi: 10.1177/01926233221129233. Epub 2022 Oct 6.
Extraintestinal pathogenic expressing cytotoxic necrotizing factor (CNF) 1 and 2 virulence factors is a rarely reported cause of acute, fatal necrohemorrhagic pneumonia in canines. A review of cases of necrohemorrhagic pneumonia in beagles at our facility between 2013 and 2021 revealed 21 dogs that died or were euthanized after acute onset lethargy, dyspnea, and hemorrhage. Some affected animals had recently been transported to the facility. In all dogs, lung lobes were discolored dark red and consolidated. Histologic lesions in 17 of these included alveolar necrosis, hemorrhage, edema, fibrin, acute inflammation, and intralesional colonies of bacilli. Lung was cultured for 10 dogs with isolated and CNF1 identified by virulence factor PCR in 7 of those. Based on these findings, extraintestinal should be considered an important cause of acute fatal necrohemorrhagic pneumonia in purpose-bred beagle research dogs and may be associated with a recent history of transport.
表达细胞毒性坏死因子（CNF）1 和 2 毒力因子的肠外致病性 是犬急性致命性坏死性出血性肺炎的罕见病因。对 2013 年至 2021 年期间我们机构内比格犬坏死性肺炎病例的回顾显示，21 只犬在急性发作性嗜睡、呼吸困难和出血后死亡或被安乐死。一些受影响的动物最近被运送到该机构。所有犬的肺叶均呈暗红色和实变。其中 17 只动物的组织学病变包括肺泡坏死、出血、水肿、纤维蛋白、急性炎症和细菌的病灶内菌落。对 10 只犬进行了肺部培养，其中 7 只犬通过毒力因子 PCR 鉴定出 和 CNF1。基于这些发现，应将肠外 视为目的培育比格犬研究犬急性致命性坏死性出血性肺炎的重要病因，并且可能与近期的运输史有关。
