as an emerging model in food and nutrition research: importance of standardizing base diet.

As a model organism that has helped revolutionize life sciences, has been increasingly used in nutrition research. Here we explore the tradeoffs between pros and cons of its use as a dietary model based primarily on literature review from the past decade. We first provide an overview of its experimental strengths as an animal model, focusing on lifespan and healthspan, behavioral and physiological phenotypes, and conservation of key nutritional pathways. We then summarize recent advances of its use in nutritional studies, e.g. food preference and feeding behavior, sugar status and metabolic reprogramming, lifetime and transgenerational nutrition tracking, and diet-microbiota-host interactions, highlighting cutting-edge technologies originated from or developed in . We further review current challenges of using as a nutritional model, followed by in-depth discussions on potential solutions. In particular, growth scales and throughputs, food uptake mode, and axenic culture of are appraised in the context of food research. We also provide perspectives for future development of chemically defined nematode food ("NemaFood") for , which is now widely accepted as a versatile and affordable in vivo model and has begun to show transformative potential to pioneer nutrition science.