Saxena U, Nagpurkar A, Coe J E, Mookerjea S
Biochem Cell Biol. 1987 May;65(5):438-43. doi: 10.1139/o87-056.
We have previously shown the effect of phosphorylcholine-binding proteins from rat (PCBP) and rabbit (CRP) on the precipitation of serum lipoproteins by heparin in presence of Ca2+. The present paper describes the effect of a phosphorylcholine-binding protein from the female Syrian hamster (FP) on the lipoprotein precipitation reaction. The precipitation of lipoproteins by heparin was lower in assays using female hamster serum in which FP is a prominent protein, compared with assays with male serum in which FP is present in very low concentration. Depletion of FP from female serum resulted in increased lipoprotein precipitation. The addition of purified FP to assays using human very low density lipoprotein (VLDL) inhibited the precipitation reaction. The precipitation of lipoproteins was also examined using serum from male hamsters treated with diethylstilbestrol and female hamsters treated with testosterone, treatments known to modulate the levels of FP. Results indicate an inverse relationship between serum FP levels from normal and hormone-treated hamsters and the precipitation of lipoproteins from their serum. The partially desialylated FP when added to precipitation assays using human VLDL resulted in reduced inhibition of VLDL precipitation.
我们之前已经展示了来自大鼠的磷酸胆碱结合蛋白（PCBP）和来自兔子的磷酸胆碱结合蛋白（CRP）在Ca2+存在的情况下对肝素沉淀血清脂蛋白的作用。本文描述了来自雌性叙利亚仓鼠的磷酸胆碱结合蛋白（FP）对脂蛋白沉淀反应的作用。与使用FP浓度极低的雄性血清进行的检测相比，在使用雌性仓鼠血清（其中FP是一种主要蛋白质）进行的检测中，肝素对脂蛋白的沉淀作用较低。从雌性血清中去除FP会导致脂蛋白沉淀增加。向使用人极低密度脂蛋白（VLDL）进行的检测中添加纯化的FP会抑制沉淀反应。还使用用己烯雌酚处理的雄性仓鼠血清和用睾酮处理的雌性仓鼠血清检测了脂蛋白的沉淀，已知这些处理会调节FP的水平。结果表明，正常和激素处理的仓鼠血清中FP水平与它们血清中脂蛋白的沉淀呈负相关。当将部分去唾液酸化的FP添加到使用人VLDL的沉淀检测中时，会导致对VLDL沉淀的抑制作用降低。
