Infrared Spectroscopy Laboratory, Research and Development Institute R&DI, University of Vale do Paraíba - Univap. Shishima Hifumi Avenue, 2911, 12244-000, São Jose dos Campos, São Paulo, Brazil.
Photobiology Applied to Health (PhotoBios) - Research and Development Institute - R&DI, University of Vale do Paraiba, Univap. Shishima Hifumi Avenue 2911, 12244-000, São José dos Campos, São Paulo, Brazil.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jan 15;285:121916. doi: 10.1016/j.saa.2022.121916. Epub 2022 Sep 24.
Alternative therapies against pathogens are under intense investigation because of their increasing resistance to antibiotics. Photodynamic therapy (PDT) is one such alternative that has shown promising results. However, for the widespread use of PDT, it is essential to decipher underlying mechanisms, so as to improve PDT's therapeutic applications. Because of this, we have studied biochemical changes in pathogen Pseudomonas aeruginosa, a medically important bacteria that has developed antibiotic resistance, after PDT with curcumin photosensitizer. Results show a drastic decrease in α-helix protein and increased disordered and β-sheet secondary structure proteins in P. Aeruginosa post-PDT compared to control. Interestingly, these biochemical changes differ from PDT of pathogens Leishmania braziliensis and Leishmania major with photosensitizer methylene blue. This observation underlines the need for extensive studies on PDT of different pathogens to understand mechanisms of action and develop better PDT strategies.
由于病原体对抗生素的耐药性不断增强，针对病原体的替代疗法正受到强烈关注。光动力疗法（PDT）就是这样一种替代疗法，已显示出良好的效果。然而，为了广泛应用 PDT，必须破译潜在的机制，以改善 PDT 的治疗应用。基于此，我们研究了在 PDT 应用姜黄素光敏剂后，具有抗生素耐药性的医学上重要细菌铜绿假单胞菌的生化变化。结果表明，与对照相比，铜绿假单胞菌在 PDT 后α-螺旋蛋白急剧减少，无序和β-折叠二级结构蛋白增加。有趣的是，这些生化变化与光敏剂亚甲蓝的利什曼原虫和利什曼原虫 PDT 不同。这一观察结果强调了需要对不同病原体的 PDT 进行广泛研究，以了解作用机制并开发更好的 PDT 策略。
