São Carlos Institute of Physics, University of São Paulo, São Carlos, Brazil.
Department of Biomedical Engineering, College of Engineering, TX A&M University, College Station, TX, USA.
Biofouling. 2023 May-Jul;39(6):606-616. doi: 10.1080/08927014.2023.2241385. Epub 2023 Aug 3.
The inactivation of biofilm is a major challenge, as biofilms are less responsive to conventional treatments and responsible for persistent infections. This has led to the investigation of alternative approaches for biofilm control such as photodynamic (PDI) and sonodynamic (SDI) inactivation. The combination of them, known as Sonophotodynamic Inactivation (SPDI), has improved the effectiveness of the process. Curcumin, a well-established photosensitizer, has been identified as a potential sonosensitizer. This study evaluated the most effective combination for SPDI against biofilms , varying curcumin concentrations and ultrasound intensities. The results indicated that the inactivation was directly proportional to the curcumin concentration. Using curcumin 120 µM and 3.0 W.cm of ultrasound intensity, SPDI demonstrated the highest and the best synergistic results, equivalent to 6.9 ± 2.1 logs of reduction. PDI reduced 0.7 ± 0.9 log and SDI had no effect. In conclusion, SPDI with curcumin is a promising approach for biofilm inactivation.
生物膜的失活是一个主要挑战，因为生物膜对常规治疗的反应性较低，是持续性感染的罪魁祸首。这导致人们研究替代方法来控制生物膜，例如光动力（PDI）和声动力（SDI）失活。将它们结合起来，称为声动力光灭活（SPDI），提高了该过程的有效性。姜黄素作为一种成熟的光敏剂，已被确定为一种潜在的声敏剂。本研究评估了针对生物膜的 SPDI 的最有效组合，改变了姜黄素浓度和超声强度。结果表明，失活与姜黄素浓度成正比。使用 120µM 的姜黄素和 3.0 W.cm 的超声强度，SPDI 表现出最高和最佳的协同效果，相当于减少了 6.9±2.1 对数。PDI 减少了 0.7±0.9 对数，SDI 没有效果。总之，使用姜黄素的 SPDI 是一种有前途的生物膜失活方法。
