MYL4 调控区域的单体型与猪肌肉纤维大小有关。

Haplotypes within the regulatory region of MYL4 are associated with pig muscle fiber size.

机构信息

College of Animal Science, Tibet Agriculture and Animal Husbandry College, Linzhi, Tibet 860000, China; The Provincial and Ministerial Co-founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Linzhi, Tibet 860000, China.

College of Animal Sciences and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450000, China.

出版信息

Gene. 2023 Jan 20;850:146934. doi: 10.1016/j.gene.2022.146934. Epub 2022 Oct 3.

DOI:10.1016/j.gene.2022.146934

PMID:36202278

Abstract

Our previous transcriptomic study identified MYL4 (myosin light chain 4) to be associated with muscle development and growth in pigs. In this study, we aimed to investigate the genetic variation of MYL4 and determine the effect of these variations in regulating MYL4 expression and muscle fiber size. After screening the regulatory region of MYL4 in Large White pigs, we identified nine completely linked single nucleotide polymorphisms within the MYL4 regulatory region, which showed two haplotypes (H1 and H2). And in MYL4 may affect the activity of the promoter region and regulate the traits of porcine muscle. The results of Western blotting and qRT-PCR showed that haplotype H2 significantly (p < 0.01) increased the relative mRNA and protein expression of MYL4 gene in pig LD tissues, and tissue sections also showed the number of genotype H2H2 Significantly higher than genotype H1H1, in conclusion, our results suggest that MYL4 may promote muscle growth and development and can affect the transcriptional activity of MYL4 through haplotype H1 and haplotype H2.

摘要

我们之前的转录组学研究表明 MYL4（肌球蛋白轻链 4）与猪的肌肉发育和生长有关。在这项研究中，我们旨在研究 MYL4 的遗传变异，并确定这些变异在调节 MYL4 表达和肌纤维大小中的作用。在筛选大白猪 MYL4 的调控区后，我们在 MYL4 调控区鉴定出了 9 个完全连锁的单核苷酸多态性，它们显示出两种单倍型（H1 和 H2）。并且在 MYL4 可能影响启动子区域的活性并调节猪肌肉的性状。Western blotting 和 qRT-PCR 的结果表明，单倍型 H2 显著（p < 0.01）增加了猪 LD 组织中 MYL4 基因的相对 mRNA 和蛋白表达，组织切片还显示基因型 H2H2 的数量明显高于基因型 H1H1，综上所述，我们的结果表明 MYL4 可能促进肌肉生长和发育，并可以通过单倍型 H1 和单倍型 H2 影响 MYL4 的转录活性。