Ye Yourong, Wu Guoxin, Wang Haoqi, Duan Mengqi, Shang Peng, Chamba Yangzom
College of Animal Science, Tibet Agriculture and Animal Husbandry College, Linzhi 860000, China.
The Provincial and Ministerial Co-Founded Collaborative Innovation Center for R&D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Linzhi 860000, China.
Animals (Basel). 2024 May 2;14(9):1370. doi: 10.3390/ani14091370.
Muscle growth stands as a pivotal economic trait within pig production, governed by a complex interplay of multiple genes, each playing a role in its quantitative manifestation. Understanding the intricate regulatory mechanisms of porcine muscle development is crucial for enhancing both pork yield and quality. This study used the GSE99749 dataset downloaded from the GEO database, conducting a detailed analysis of the RNA-seq results from the longissimus dorsi muscle (LD) of Tibetan pigs (TP), Wujin pigs (WJ) and large white pigs (LW) at 60 days of gestation, representing diverse body sizes and growth rates. Comparative analyses between TPvsWJ and TPvsLW, along with differential gene expression (DEG) analysis, functional enrichment analysis, and protein-protein interaction (PPI) network analysis, revealed 1048 and 1157 significantly differentially expressed genes ( < 0.001) in TPvsWJ and TPvsLW, respectively. With stricter screening criteria, 37 DEGs were found to overlap between the 2 groups. PPI analysis identified , , and as the three core genes. This article focuses on exploring the gene. Molecular-level experimental validation, through overexpression and interference of the gene combined with EDU staining experiments, demonstrated that overexpression of significantly promoted the proliferation of porcine skeletal muscle satellite cells (PSMSC), while interference with inhibited their proliferation. Furthermore, by examining the effects of overexpressing and interfering with the gene on the muscle hypertrophy marker gene and the muscle degradation marker gene, the pivotal role of the gene in promoting muscle growth and preventing muscle degradation was further confirmed. These findings offer a new perspective on the molecular mechanisms behind porcine muscle growth and development, furnishing valuable data and insights for muscle biology research.
肌肉生长是生猪生产中的一个关键经济性状，受多个基因复杂的相互作用所调控，每个基因在其数量表现中都发挥着作用。了解猪肌肉发育的复杂调控机制对于提高猪肉产量和质量至关重要。本研究使用从GEO数据库下载的GSE99749数据集，对藏猪（TP）、武进猪（WJ）和大白猪（LW）妊娠60天时背最长肌（LD）的RNA测序结果进行了详细分析，这些猪代表了不同的体型和生长速度。TP与WJ以及TP与LW之间的比较分析，以及差异基因表达（DEG）分析、功能富集分析和蛋白质-蛋白质相互作用（PPI）网络分析显示，TP与WJ和TP与LW中分别有1048个和1157个显著差异表达基因（<0.001）。采用更严格的筛选标准，发现两组之间有37个差异基因重叠。PPI分析确定 、 和 为三个核心基因。本文重点探索 基因。通过对 基因进行过表达和干扰，并结合EDU染色实验进行分子水平的实验验证，结果表明 基因的过表达显著促进了猪骨骼肌卫星细胞（PSMSC）的增殖，而对 的干扰则抑制了它们的增殖。此外，通过检测过表达和干扰 基因对肌肉肥大标记基因 和肌肉降解标记基因 的影响，进一步证实了 基因在促进肌肉生长和防止肌肉降解中的关键作用。这些发现为猪肌肉生长发育背后的分子机制提供了新的视角，为肌肉生物学研究提供了有价值的数据和见解。
