Ishibashi T, Harada Y, Harada S, Yamada H, Takamoto M, Sugiyama K
Jpn J Exp Med. 1978 Jun;48(3):227-32.
The mode of action of BCG, strain Japan was investigated using the immune response to sheep red blood cells (SRBC) as an indicator system. When SRBC were injected into the BCG-primed foot pad, the direct plaque forming cells (PFC) and the effector cells responsible for delayed type hypersensitivity (PTH) were produced in various lymphoid organs widely distributed. Furthermore, bacterial counts in the draining popliteal lymph node and the spleen in the mice inoculated with BCG into the hind foot pad suggested that a local infection with BCG has spread to a generalized systemic infection of lymphoid tissues with time. Enhancement of DTH response to SRBC was induced when the mice previously infected with BCG were inmunized with SRBC mixed with purified protein derivative (PPD). These findings suggested that nonspecific augmentation of immune response with BCG was due to a generalized systemic activation of lymphoid system by BCG infection and the long lasting effect of immunopotentiation with BCG was due to persisting BCG infection in the lymphoid tissues.
以对绵羊红细胞（SRBC）的免疫反应作为指标系统，研究了日本卡介苗（BCG）株的作用方式。当将SRBC注入经卡介苗致敏的足垫时，在广泛分布的各种淋巴器官中产生了直接斑块形成细胞（PFC）和负责迟发型超敏反应（PTH）的效应细胞。此外，在后足垫接种卡介苗的小鼠中，引流腘窝淋巴结和脾脏中的细菌计数表明，随着时间的推移，卡介苗的局部感染已扩散至淋巴组织的全身性感染。当先前感染卡介苗的小鼠用与纯化蛋白衍生物（PPD）混合的SRBC免疫时，诱导了对SRBC的迟发型超敏反应增强。这些发现表明，卡介苗对免疫反应的非特异性增强是由于卡介苗感染引起的淋巴系统全身性激活，而卡介苗免疫增强的持久作用是由于淋巴组织中持续存在的卡介苗感染。
