Kato K, Yamamoto K, Kimura T, Azuma I, Askenase P W
J Immunol. 1984 Jun;132(6):2790-5.
We showed previously that antigen-nonspecific suppressor T cells induced by i.v. injection of heat-killed bacillus Calmette-Guérin (BCG) were involved in suppression of delayed-type hypersensitivity (DTH). We suggested that the adjuvant portion of BCG might be involved in the induction of these cells. In this report, we show that BCG cell wall-induced DTH responses in mice pretreated with muramyl dipeptide (MDP), a minimum adjuvant constituent of BCG, were suppressed nonspecifically. In addition, we show that pretreatment with tuberculin active peptide (TAP), the antigenic peptide from Mycobacterium tuberculosis, induces antigen-specific suppression of DTH responses. In both instances, suppression was shown to be due to non-adherent cells that act to inhibit elicitation of DTH. Furthermore, using the macrophage migration inhibition assay, an in vitro correlate of DTH, we found that antigen-nonspecific and antigen-specific suppressor T cells were induced by the injection of MDP and TAP, respectively. Thus, suppressor T cells induced by the adjuvant and antigen portions of BCG may act by interfering with the lymphokine-dependent mechanisms by which DTH effector T cells elicit DTH.
我们先前表明，静脉注射热灭活卡介苗（BCG）诱导的抗原非特异性抑制性T细胞参与迟发型超敏反应（DTH）的抑制。我们认为卡介苗的佐剂部分可能参与这些细胞的诱导。在本报告中，我们表明，在经胞壁酰二肽（MDP，卡介苗的最小佐剂成分）预处理的小鼠中，卡介苗细胞壁诱导的DTH反应受到非特异性抑制。此外，我们表明，用结核菌素活性肽（TAP，来自结核分枝杆菌的抗原肽）预处理可诱导DTH反应的抗原特异性抑制。在这两种情况下，抑制均显示是由于非黏附细胞抑制DTH的激发。此外，使用DTH的体外相关指标巨噬细胞迁移抑制试验，我们发现分别注射MDP和TAP可诱导抗原非特异性和抗原特异性抑制性T细胞。因此，卡介苗的佐剂和抗原部分诱导的抑制性T细胞可能通过干扰DTH效应T细胞引发DTH的淋巴因子依赖性机制发挥作用。
