Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.
Faculty of Agriculture, Kyushu University, Fukuoka, 819-0385, Japan.
Sci Rep. 2022 Oct 6;12(1):16707. doi: 10.1038/s41598-022-20838-9.
In our previous study, enterohepatic 12α-hydroxylated (12α) bile acid (BA) levels were found to be correlated with hepatic triacylglycerol concentration in rats fed high-fat (HF) diet. Since BA composition is diverse depending on animal species, we evaluated whether such a relationship is applicable in mice in response to an HF diet. C57BL/6JJmsSLC (B6) male mice were fed HF diet for 13 weeks and analyzed for triacylglycerol, cholesterol, oxysterols, and other metabolites in the liver. The BA composition was determined in the liver, small intestinal contents, portal plasma, aortic plasma, and feces. Neutral sterols were also measured in the feces. The ratio of 12α BA/non-12 BA increased in the liver, portal plasma, small intestinal contents, and feces of HF-fed B6 mice. Moreover, a positive correlation was observed between the ratio of fecal 12α BAs/non-12 BAs and hepatic triacylglycerol concentration. The concentration of 7α-hydroxycholesterol was increased in the liver of HF-fed B6 mice, whereas no increase was observed in the hepatic expression of cytochrome P450 family 7 subfamily A member 1. The present study showed that the ratio of 12α BA/non-12 BA in feces is closely associated with hepatic triacylglycerol accumulation in B6 mice fed HF diet.
在我们之前的研究中，发现高脂肪（HF）饮食喂养的大鼠的肠肝 12α-羟化（12α）胆汁酸（BA）水平与肝三酰甘油浓度相关。由于 BA 组成因动物种类而异，我们评估了这种关系是否适用于 HF 饮食喂养的小鼠。C57BL/6JJmsSLC（B6）雄性小鼠用 HF 饮食喂养 13 周，分析肝中三酰甘油、胆固醇、氧化固醇和其他代谢物。测定肝、小肠内容物、门静脉血浆、主动脉血浆和粪便中的 BA 组成。粪便中也测定中性固醇。HF 喂养的 B6 小鼠的肝、门静脉血浆、小肠内容物和粪便中的 12α BA/非-12 BA 比值增加。此外，粪便中 12α BAs/非-12 BAs 的比值与肝三酰甘油浓度呈正相关。HF 喂养的 B6 小鼠肝中 7α-羟胆固醇浓度增加，而肝 CYP450 家族 7 亚家族 A 成员 1 的表达没有增加。本研究表明，HF 饮食喂养的 B6 小鼠粪便中 12α BA/非-12 BA 比值与肝三酰甘油积累密切相关。
