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在接种SARS-CoV-2 mRNA疫苗后，母乳中富含刺突蛋白特异性T细胞。

Spike-specific T cells are enriched in breastmilk following SARS-CoV-2 mRNA vaccination.

作者信息

Armistead Blair, Jiang Yonghou, Carlson Marc, Ford Emily S, Jani Saumya, Houck John, Wu Xia, Jing Lichen, Pecor Tiffany, Kachikis Alisa, Yeung Winnie, Nguyen Tina, Minkah Nana, Larsen Sasha E, Coler Rhea N, Koelle David M, Harrington Whitney E

机构信息

Center for Global Infectious Disease Research, Seattle Children's Research Institute; Seattle, WA, USA.

Research Scientific Computing, Enterprise Analytics, Seattle Children's Research Institute; Seattle, WA, USA.

出版信息

medRxiv. 2022 Sep 28:2021.12.03.21267036. doi: 10.1101/2021.12.03.21267036.

DOI:10.1101/2021.12.03.21267036
PMID:36203549
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9536058/
Abstract

UNLABELLED

Human breastmilk is rich in T cells; however, their specificity and function are largely unknown. We compared the phenotype, diversity, and antigen specificity of T cells in the breastmilk and peripheral blood of lactating individuals who received SARS-CoV-2 mRNA vaccination. Relative to blood, breastmilk contained higher frequencies of T effector and central memory populations that expressed mucosal-homing markers. T cell receptor (TCR) sequence overlap was limited between blood and breastmilk. Overabundan t breastmilk clones were observed in all individuals, were diverse, and contained CDR3 sequences with known epitope specificity including to SARS-CoV-2 Spike. Spike-specific TCRs were more frequent in breastmilk compared to blood and expanded in breastmilk following a third mRNA vaccine dose. Our observations indicate that the lactating breast contains a distinct T cell population that can be modulated by maternal vaccination with potential implications for infant passive protection.

ONE-SENTENCE SUMMARY: The breastmilk T cell repertoire is distinct and enriched for SARS-CoV-2 Spike-specificity after maternal mRNA vaccination.

摘要

未标注

人母乳富含T细胞；然而，它们的特异性和功能在很大程度上尚不清楚。我们比较了接受SARS-CoV-2 mRNA疫苗接种的哺乳期个体母乳和外周血中T细胞的表型、多样性和抗原特异性。相对于血液，母乳中表达黏膜归巢标志物的T效应细胞和中央记忆细胞群体的频率更高。血液和母乳之间的T细胞受体（TCR）序列重叠有限。在所有个体中均观察到母乳中克隆过度丰富，它们具有多样性，并且包含具有已知表位特异性（包括针对SARS-CoV-2刺突蛋白）的CDR3序列。与血液相比，母乳中刺突蛋白特异性TCR更频繁，并且在第三次mRNA疫苗接种后在母乳中扩增。我们的观察结果表明，哺乳期乳房含有独特的T细胞群体，可通过母体疫苗接种进行调节，这对婴儿被动保护具有潜在影响。

一句话总结

母体mRNA疫苗接种后，母乳T细胞库独特且富含SARS-CoV-2刺突蛋白特异性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7234/9536058/615869c7abd8/nihpp-2021.12.03.21267036v2-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7234/9536058/aca6c2dbf3cb/nihpp-2021.12.03.21267036v2-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7234/9536058/c4e65e0dc54e/nihpp-2021.12.03.21267036v2-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7234/9536058/6e270ecc3b5f/nihpp-2021.12.03.21267036v2-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7234/9536058/615869c7abd8/nihpp-2021.12.03.21267036v2-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7234/9536058/aca6c2dbf3cb/nihpp-2021.12.03.21267036v2-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7234/9536058/c4e65e0dc54e/nihpp-2021.12.03.21267036v2-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7234/9536058/6e270ecc3b5f/nihpp-2021.12.03.21267036v2-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7234/9536058/615869c7abd8/nihpp-2021.12.03.21267036v2-f0004.jpg

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Secretory IgA and T cells targeting SARS-CoV-2 spike protein are transferred to the breastmilk upon mRNA vaccination.
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TCR meta-clonotypes for biomarker discovery with enabled identification of public, HLA-restricted clusters of SARS-CoV-2 TCRs.利用 TCR 元克隆型进行生物标志物发现， 能够识别 SARS-CoV-2 TCR 的公共、HLA 限制簇。
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