Zhang Jiankang, Xia Xueping, Zhu Ying, Lian Zitong, Tian Haifeng, Xiao Hanbing, Hu Qiaomu
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China.
School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, China.
Front Mol Biosci. 2022 Sep 20;9:974348. doi: 10.3389/fmolb.2022.974348. eCollection 2022.
Farmed chinese giant salamander () was an important distinctive economically amphibian that exhibited male-biased sexual size dimorphism. and genes antagonize each other in Wnt4 signal pathway to regulate mammalian gonadal differentiation has been demonstrated. However, their expression profile and function in are unclear In this study, we firstly characterized and genes expression in developing gonad. Results showed that expression level was higher in testes than in ovaries and increased from 1 to 6 years while expression was higher in ovaries than in testes. hybridization assay showed that both and genes expressed at 62 dpf in undifferentiated gonad, and gene was mainly expressed in spermatogonia and sertoli cells in testis while strong positive signal of was detected in granular cell in ovary. During sex-reversal, expression was significantly higher in reversed testes and normal testes than in ovaries, and opposite expression pattern was detected for When FH535 was used to inhibit Wnt/β-catenin pathway, expression of , was down-regulated. Conversely, expression of and were up-regulated. Furthermore, when were knocked down using RNAi technology, respectively. We observed that female biased genes were down regulated in ovary primordial cells after was knocked down, while the opposite expression profile was observed in testis primordial cells after was knocked down. These results suggested that and played an antagonistic role to regulate sex differentiation in the process of the gonadal development and provided a foundation for further functional characterizations. The data also provided basic information for genome editing breeding to improve the Chinese giant salamander farming industry.
养殖中国大鲵是一种重要的具有独特经济价值的两栖动物，表现出雄性偏向的两性异形。Wnt4信号通路中 基因和 基因相互拮抗以调节哺乳动物性腺分化已得到证实。然而，它们在中国大鲵中的表达谱和功能尚不清楚。在本研究中，我们首先对发育中的性腺中 基因和 基因的表达进行了表征。结果表明， 在睾丸中的表达水平高于卵巢，且从1岁到6岁有所增加，而 在卵巢中的表达高于睾丸。原位杂交分析表明， 基因和 基因在62日龄未分化性腺中均有表达， 基因主要在睾丸的精原细胞和支持细胞中表达，而在卵巢的颗粒细胞中检测到 的强阳性信号。在性反转过程中， 基因在反转睾丸和正常睾丸中的表达明显高于卵巢，而 基因则呈现相反的表达模式。当使用FH535抑制Wnt/β-连环蛋白信号通路时， 基因、 基因的表达下调。相反， 基因和 基因的表达上调。此外，当分别使用RNAi技术敲低 基因和 基因时。我们观察到，敲低 基因后卵巢原始细胞中雌性偏向基因下调，而敲低 基因后睾丸原始细胞中则观察到相反的表达谱。这些结果表明， 基因和 基因在性腺发育过程中对性别分化起拮抗作用，为进一步的功能表征奠定了基础。这些数据也为基因组编辑育种改善中国大鲵养殖业提供了基础信息。
