Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei 430223, China.
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei 430223, China.
Genomics. 2019 Dec;111(6):1351-1359. doi: 10.1016/j.ygeno.2018.09.007. Epub 2018 Sep 20.
We characterized the Andrias davidianus T-box 1 (Tbx1) gene. Tbx1 expression was high in testis and low in other examined tissues. Immunohistochemistry detected tbx1 expression in somatic and germ cells 62 days post-hatching (dph), prior to gonad differentiation. At 210 dph, after gonad differentiation, tbx1 was expressed in spermatogonia and testis somatic cells and in granulosa cells in ovary. Tbx1 expression was up-regulated in ovary after high temperature treatment. In the neomale, tbx1 expression showed a similar profile to normal males, and vice-versa for genetic male. Over-expression of tbx1 in females after injection of TBX1 protein down-regulated the female-biased genes cyp19a and foxl2 and up-regulated the male-biased amh gene. When tbx1 was knocked down by tbx1/siRNA, cyp19a and foxl2 expression was up-regulated, and expression of amh, cyp26a, dmrt1, and wt1 was down-regulated. Results suggest that tbx1 influenced sex-related gene expression and participates in regulation of A. davidianus testis development.
我们对大鲵 T -box 1（Tbx1）基因进行了特征分析。Tbx1 在睾丸中表达较高，在其他检查组织中表达较低。免疫组织化学检测到 tbx1 在孵化后 62 天（dph）的体细胞和生殖细胞中表达，此时性腺尚未分化。在 210 dph 时，在性腺分化后，tbx1 在精原细胞和睾丸体细胞以及卵巢中的颗粒细胞中表达。高温处理后，卵巢中 tbx1 的表达上调。在新雄鱼中，tbx1 的表达与正常雄鱼相似，而遗传雄鱼则相反。在注射 TBX1 蛋白后，tbx1 在雌性鱼中的过表达下调了雌性偏向基因 cyp19a 和 foxl2，并上调了雄性偏向基因 amh。当 tbx1 被 tbx1/siRNA 敲低时，cyp19a 和 foxl2 的表达上调，amh、cyp26a、dmrt1 和 wt1 的表达下调。结果表明，tbx1 影响性别相关基因的表达，并参与调控大鲵睾丸发育。
