Munakata H, Hsu C C, Kodama C, Aikawa J, Sakurada M, Ototani N, Isemura M, Yosizawa Z, Hayashi N
Biochim Biophys Acta. 1987 Sep 11;925(3):325-31. doi: 10.1016/0304-4165(87)90198-x.
We prepared dermatan sulfate specimens from various porcine tissues, and compared their heparin cofactor II-mediated thrombin-inhibitory activities and chemical natures, including disaccharide composition. Electrophoresis of the specimens on cellulose acetate membrane indicated that spleen dermatan sulfate was the most acidic of the dermatan sulfates prepared from the various porcine tissues. Analysis of the disaccharide units of the dermatan sulfate specimens by high-performance liquid chromatography revealed that spleen dermatan sulfate was rich in 4,6-di-O-sulfated N-acetylgalactosamine residues as compared with those of the other tissues. Spleen dermatan sulfate exhibited the highest thrombin-inhibitory activity, which may be related to its high content of the disulfated N-acetylgalactosamine residue.
我们从各种猪组织中制备了硫酸皮肤素标本，并比较了它们的肝素辅因子II介导的凝血酶抑制活性和化学性质，包括二糖组成。在醋酸纤维素膜上对标本进行电泳表明，脾脏硫酸皮肤素是从各种猪组织中制备的硫酸皮肤素中酸性最强的。通过高效液相色谱法对硫酸皮肤素标本的二糖单元进行分析发现，与其他组织相比，脾脏硫酸皮肤素富含4,6-二-O-硫酸化的N-乙酰半乳糖胺残基。脾脏硫酸皮肤素表现出最高的凝血酶抑制活性，这可能与其高含量的二硫酸化N-乙酰半乳糖胺残基有关。
