Tang Shuyi, Tezuka Moeko, Otsuka Hinata, Sato Ryoichi
Graduate School of Bio-Application and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan.
Arch Insect Biochem Physiol. 2023 Jan;112(1):e21975. doi: 10.1002/arch.21975. Epub 2022 Oct 7.
The existence of an extracellular signaling pathway that mediates nodule formation, a cell-mediated immune response, has been reported in Bombyx mori larvae. In this pathway, C-type lectins and the hemolymph serine proteinase BmHP-8 function in pathogen associated molecular pattern (PAMPs) recognition and signaling transduction. However, which molecule elicits the cellular response at the end of the pathway is unknown. In this study, the Toll ligand Bombyx mori Spätzel1 was shown to be involved in the pathway by applying anit-Spätzel1 antiserum in an in vitro nodule-like aggregate formation assay and an in vivo nodule formation assay.
在家蚕幼虫中，已经报道了一条介导结节形成（一种细胞介导的免疫反应）的细胞外信号通路的存在。在该通路中，C型凝集素和血淋巴丝氨酸蛋白酶BmHP-8在病原体相关分子模式（PAMPs）识别和信号转导中发挥作用。然而，在该通路末端引发细胞反应的是哪种分子尚不清楚。在本研究中，通过在体外结节样聚集体形成试验和体内结节形成试验中应用抗Spätzel1抗血清，表明Toll配体家蚕Spätzel1参与了该通路。
