Department of Toxicology and Sanitary Chemistry, School of Public Health, Tianjin Medical University, Tianjin, PR China.
Tianjin Key Laboratory of Environment, Nutrition and Public Health, Tianjin Medical University, Tianjin, PR China.
J Obstet Gynaecol. 2022 Nov;42(8):3605-3610. doi: 10.1080/01443615.2022.2130205. Epub 2022 Oct 7.
Sepsis caused by methicillin-resistant coagulase-negative staphylococci (MRCoNS) seriously affects the morbidity and mortality of neonates. However, the hand carriage and genotypic diversity of MRCoNS within surgical staff remain unclear in China. In the study, antimicrobial susceptibility tests and genotypic characterisation were applied to MRCoNS. One hundred and one samples were collected from the hands of gynaecological surgical staff. Eighty staphylococcal isolates were identified, of which 75 (94%) were resistant to at least one antibiotic. gene was determined in 50 (62.5%) staphylococcal isolates. Panton-Valentine leukocidin () and genes were determined in 17 (21%) and 12 (15%) staphylococcal isolates, respectively. About 52% of staphylococci carried SCC IV and V, followed by SCC type I, II, and III (38%). In addition, two new STs types were assigned as ST844 and ST845. The high level of hand MRCoNS colonisation rate in gynaecological surgical staff is of concern, and hand hygiene management should be emphasised among surgical assistants.Impact statement Coagulase-negative staphylococci (CoNS) are the predominant cause of neonatal sepsis. Exposure to antimicrobial-resistant CoNS might put neonates at increased risk of infection. However, little is known about the carriage and genetic diversity of methicillin-resistant CoNS (MRCoNS) of gynaecological surgeons and surgical assistants. This is the first study on the molecular characterisation of MRCoNS recovered from gynaecological surgeons and surgical assistants in China. MRCoNS carriage rate in surgical assistants was significantly higher than in surgeons. Seventy-five (94%) coagulase-negative staphylococci were resistant to at least one antibiotic. SCC I, II and III was the dominant types identified in MRCoNS that were recovered from surgical staff. Fifty (62.5%) staphylococcal isolates that were recovered from surgical staff harboured the gene. Pathogenic clones of MRCoNS were disseminated in surgical staff, and half of -positive harboured the gene. The high level of hand MRCoNS colonisation rate among gynaecological surgical staff is of concern. The alarming outcome of this study suggested that hygiene measures should be emphasised among gynaecological surgical assistants.
耐甲氧西林凝固酶阴性葡萄球菌（MRCoNS）引起的败血症严重影响新生儿的发病率和死亡率。然而，中国外科医护人员携带和 MRCoNS 的基因多态性尚不清楚。本研究应用抗菌药物敏感性试验和基因特征分析对 MRCoNS 进行了研究。从妇科手术医护人员的手上采集了 101 份样本。共鉴定出 80 株葡萄球菌分离株，其中 75 株（94%）对至少一种抗生素耐药。在 50 株（62.5%）葡萄球菌分离株中检测到 基因。在 17 株（21%）和 12 株（15%）葡萄球菌分离株中分别检测到杀白细胞素（Panton-Valentine leukocidin，）和 基因。大约 52%的葡萄球菌携带 SCC IV 和 V，其次是 SCC 型 I、II 和 III（38%）。此外，还鉴定出两种新的 ST 型，分别为 ST844 和 ST845。妇科手术医护人员手部耐甲氧西林凝固酶阴性葡萄球菌的高定植率令人担忧，应强调手术助手的手部卫生管理。
