Beppu M, Ochiai H, Kikugawa K
Biochim Biophys Acta. 1987 Sep 14;930(2):244-53. doi: 10.1016/0167-4889(87)90037-1.
Mouse erythrocytes modified by oxidizing agents (ADP/Fe3+, tert-butyl hydroperoxide and phenylhydrazine) were examined for recognition by autologous macrophages (cell adhesion and phagocytosis). Treatment of erythrocytes with ADP/Fe3+ resulted in lipid oxidation but no significant alterations in physical properties, and the treated cells were adherent to macrophages. When the lipid oxidation of erythrocytes was prevented by alpha-tocopherol, erythrocytes were not susceptible to macrophage adhesion, indicating that free radical reactions involving lipid oxidation are responsible for the formation or exposure of the membrane sites for the macrophage adhesion. Treatment of erythrocytes with tert-butyl hydroperoxide caused lipid oxidation and alterations in physical properties. Deformability and osmotic fragility of the treated cells were markedly low. Not only cell adhesion but subsequent phagocytosis of the treated cells by macrophages were observed. A similar effect was observed on treatment of erythrocytes with phenylhydrazine. The pronounced alterations in physical properties of the erythrocytes treated with tert-butyl hydroperoxide and phenylhydrazine may be responsible for the macrophage phagocytosis.
研究了经氧化剂（ADP/Fe3+、叔丁基过氧化氢和苯肼）修饰的小鼠红细胞被自体巨噬细胞识别的情况（细胞黏附和吞噬作用）。用ADP/Fe3+处理红细胞会导致脂质氧化，但物理性质无显著改变，且处理后的细胞可黏附于巨噬细胞。当用α-生育酚阻止红细胞的脂质氧化时，红细胞不易被巨噬细胞黏附，这表明涉及脂质氧化的自由基反应是巨噬细胞黏附的膜位点形成或暴露的原因。用叔丁基过氧化氢处理红细胞会导致脂质氧化和物理性质改变。处理后的细胞的变形性和渗透脆性明显降低。不仅观察到细胞黏附，还观察到巨噬细胞对处理后的细胞的后续吞噬作用。用苯肼处理红细胞也观察到类似效果。用叔丁基过氧化氢和苯肼处理的红细胞物理性质的显著改变可能是巨噬细胞吞噬作用的原因。
