prevents high-fat diet-induced non-alcoholic fatty liver disease in rats by inhibiting expression the TLR4/MyD88 and the phosphorylation of NF-κB.

CONTEXT

Franch. (Gentianaceae) is a source of the traditional Tibetan medicine, ZangYinChen, and is used to treat chronic hepatitis and many types of jaundice.

OBJECTIVE

This study explored the therapeutic effects and mechanism of on non-alcoholic fatty liver disease in diet-induced hypercholesterolaemia.

MATERIALS AND METHODS

After a week of adaptive feeding, 32 Sprague-Dawley rats were divided into four groups: (1) Control, (2) Control-S, (3) Model, and (4) Model-S. During the 12 experimental weeks, we established the Model using a high-fat diet. Control-S and Model-S were given 1.0 g/kg water extract via gavage starting in the fifth week until the end of experiment.

RESULTS

When compared with Model rats, the water extract led to a reduction in high-density lipoproteins (43.9%) and albumin (13.9%) and a decrease in total cholesterol (54.0%), triglyceride (45.6%), low-density lipoproteins (8.6%), aspartate aminotransferase (11.0%), alanine aminotransferase (15.5%), alkaline phosphatase (19.1%), total protein (6.4%), and glucose (20.8%) in serum. A reduction in three cytokines (IL-1β, IL-6, and TNFα) was detected. Histopathological examination showed that liver steatosis was significantly relieved in -treated high-fat diet rats. also caused a downregulation in the expression of TLR4 (43.2%), MyD88 (33.3%), and a decrease in phosphorylation of NF-κB.

DISCUSSION AND CONCLUSIONS

Our findings indicate that may act as a potential anti-inflammation drug via inhibition of the TLR4/MyD88/NF-κB pathway. Further and studies are needed to validate its potential in clinical medicine.