Kasai K, Teuscher C, Smith S, Matzner P, Tung K S
Biol Reprod. 1987 Jun;36(5):1085-94. doi: 10.1095/biolreprod36.5.1085.
This study provides evidence for polygenic controls of antisperm antibody levels in inbred male mice immunized with syngenic testis and epididymis. H2-linked and non-H2-linked genes were involved. Mice of H-2d haplotype were high responders, whereas those with H-2k haplotype were nonresponders; however, B10.D2/nSnJ mice (H-2d) were also nonresponders. In vitro fertilization inhibition by antisera correlated positively with the serum antisperm antibody levels, particularly with antibody of the immunoglobulin (Ig) G class. Inheritance of antibody response that inhibited in vitro fertilization (IVF) was an autosomal dominant trait, but this was not apparent for the control of antibody levels per se. Since IVF was inhibited by both IgG and fragment antigen-binding (Fab) isolated from immune sera, but not by immune IgG previously absorbed by sperm or testis, the biologic effect is antigen-specific and probably involved blockade of functional antigenic epitopes. Antisera to testis, caput sperm or cauda sperm were found to inhibit IVF to a similar degree. Inbred strains of mice that produced the highest levels of serum antisperm antibodies that inhibited IVF were A/J, SJL/J, DBA/1J and BALB/cByJ mice, and their antisera immunoprecipitated a common sperm antigen molecule of 35,000 to 40,000 Mr. In contrast, C57BL/6 and C57BL/10 mice produced significant antibody levels that had no effect on IVF, and their sera did not react with the 35,000- to 40,000-Mr peak. Moreover, among BALB/c H-2 congenic mice, only antiserum of responder BALB/cByJ (H-2d) mice immunoprecipitated the 35,000- to 40,000 Mr peak. Thus the 35,000- to 40,000-Mr protein may be of functional significance in the fertilization process.
本研究为近交系雄性小鼠经同基因睾丸和附睾免疫后抗精子抗体水平的多基因控制提供了证据。涉及H2连锁基因和非H2连锁基因。H-2d单倍型的小鼠是高反应者，而H-2k单倍型的小鼠是非反应者；然而，B10.D2/nSnJ小鼠（H-2d）也是非反应者。抗血清对体外受精的抑制作用与血清抗精子抗体水平呈正相关，尤其是与免疫球蛋白（Ig）G类抗体相关。抑制体外受精（IVF）的抗体反应的遗传是一种常染色体显性性状，但对于抗体水平本身的控制而言并非如此。由于IgG和从免疫血清中分离出的抗原结合片段（Fab）均可抑制IVF，但先前被精子或睾丸吸收的免疫IgG则无此作用，因此其生物学效应具有抗原特异性，可能涉及功能性抗原表位的阻断。发现针对睾丸、附睾头部精子或附睾尾部精子的抗血清对IVF的抑制程度相似。产生抑制IVF的血清抗精子抗体水平最高的近交系小鼠是A/J、SJL/J、DBA/1J和BALB/cByJ小鼠，它们的抗血清免疫沉淀出一种分子量为35000至40000的常见精子抗原分子。相比之下，C57BL/6和C57BL/10小鼠产生的显著抗体水平对IVF没有影响，且它们的血清不与分子量为35000至40000的峰发生反应。此外，在BALB/c H-2同源基因小鼠中，只有反应性BALB/cByJ（H-2d）小鼠的抗血清免疫沉淀出分子量为35000至40000的峰。因此，分子量为35000至40000的蛋白质可能在受精过程中具有功能意义。
