Institute of Functional Genomics, University of Montpellier, CNRS, INSERM, Montpellier, France.
Methods Mol Biol. 2023;2565:113-127. doi: 10.1007/978-1-0716-2671-9_9.
Because catecholamines secretion mainly relies on the excitable nature of adrenal chromaffin cells, monitoring their electrical activity is an essential step in assessing the adrenal medullary tissue function. The difficult access to the gland in vivo allows only population activity to be recorded in this condition. In vitro preparations allow recordings of spontaneous or evoked activity from single or multiple cells, depending on the biological samples used (dissociated chromaffin cells versus adrenal tissue preparations). In this chapter, I provide a detailed description of the techniques used for electrophysiological recordings in rodent chromaffin cells in acute adrenal slices, using the patch-clamp technique. This methodology allows preservation of the tissue integrity and detection of action potentials, synaptic activity, and secretory events; it is thus suitable for the study of adrenomedullary activity-secretion coupling.
由于儿茶酚胺的分泌主要依赖于肾上腺髓质细胞的兴奋特性，因此监测其电活动是评估肾上腺髓质组织功能的重要步骤。由于在体内难以接近该腺体，因此只能在这种情况下记录细胞群体的活动。在体外，根据使用的生物样本（分离的嗜铬细胞与肾上腺组织制剂），可以记录单个或多个细胞的自发或诱发活动。在本章中，我提供了一种详细的描述，说明了在急性肾上腺切片中使用膜片钳技术记录啮齿动物嗜铬细胞电生理记录的技术。该方法学可保持组织完整性并检测动作电位、突触活动和分泌事件；因此，它适用于研究肾上腺髓质活动-分泌偶联。
