Fathy Mohamed M, Saad Omnia A, Elshemey Wael M, Fahmy Heba M
Biophysics Department, Faculty of Science, Cairo University, 12613, Giza, Egypt.
Biophysics Department, Faculty of Science, Cairo University, 12613, Giza, Egypt.
Biochem Biophys Res Commun. 2022 Dec 3;632:100-106. doi: 10.1016/j.bbrc.2022.09.087. Epub 2022 Sep 27.
Cancer radiotherapy is one of the most effective regimens of cancer treatments, but cancer cell radioresistance remains a concern. Radiosensitizers can selectively improve the efficacy of radiotherapy and reduce inherent damage. The purpose of this work is to evaluate the effect of silica-coated iron oxide magnetic nanoparticles (SIONPs) as a radiosensitizer and compare their therapeutic effect with that of Iron oxide magnetic nanoparticles (IONPs). IONPs and SIONPs were characterized using several physical techniques such as a transmission electron microscope (TEM) and Vibrating sample magnetometer (VSM). MTT and DNA double-strand breaks (Comet) assays have been used to detect the cytotoxicity, cell viability, and DNA damage of MCF-7 cells, which were treated with different concentrations of prepared nanoparticles and exposed to an X-ray beam. In this study, an efficient radiosensitizer, SIONPs, was successfully prepared and characterized. With 0.5 Gy dose, dose enhancement factor (DEF) values of cells treated with 5 and 10 μg/ml of IONPs were 1 and 1.09, respectively, while those treated with SIONPs at these concentrations had DEF of 1.21 and 1.32, respectively. Results demonstrated that SIONPs provide a potential for improving the radiosensitivity of breast cancer.
癌症放射治疗是最有效的癌症治疗方案之一，但癌细胞的放射抗性仍然是一个令人担忧的问题。放射增敏剂可以选择性地提高放射治疗的疗效并减少固有损伤。这项工作的目的是评估二氧化硅包覆的氧化铁磁性纳米颗粒（SIONPs）作为放射增敏剂的效果，并将其治疗效果与氧化铁磁性纳米颗粒（IONPs）进行比较。使用透射电子显微镜（TEM）和振动样品磁强计（VSM）等多种物理技术对IONPs和SIONPs进行了表征。MTT和DNA双链断裂（彗星）试验已用于检测用不同浓度的制备纳米颗粒处理并暴露于X射线束的MCF-7细胞的细胞毒性、细胞活力和DNA损伤。在本研究中，成功制备并表征了一种高效的放射增敏剂SIONPs。在0.5 Gy剂量下，用5和10μg/ml的IONPs处理的细胞的剂量增强因子（DEF）值分别为1和1.09，而用这些浓度的SIONPs处理的细胞的DEF分别为1.21和1.32。结果表明，SIONPs具有提高乳腺癌放射敏感性的潜力。
