Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Department of Rheumatology, Rehabilitation & Physical Medicine, Faculty of Medicine, Assiut University, Assiut, Egypt.
Egypt J Immunol. 2022 Oct;29(4):184-194.
Rheumatoid arthritis (RA) is an autoimmune disease characterized by autoantibodies against citrullinated antigens. The anti-cyclic citrullinated peptide (Anti-CCP) test is commonly used to diagnose rheumatoid arthritis, whereas the anti-mutated citrullinated vimentin (Anti-MCV) is another anti-citrullinated antibody that reacts with mutated citrullinated vimentin. Therefore, we aimed to assess the diagnostic value of anti-MCV antibodies in RA patients and their relation to disease activity. This study included 60 RA patients and 25 normal controls. The disease activity of RA patients was assessed by disease activity score (DAS-28). ELISA was used to test patients and controls for anti-MCV and anti-CCP. The level of anti-MCV was significantly higher among patients with RA compared to the control group (1.56 ± 0.56 vs. 1.20 ± 0.19 mol/l; P< 0.001). Anti-MCV at cut-off point of > 1.2 mol/l had 76% sensitivity and 100% specificity, with an overall diagnostic accuracy of 83.2% for diagnosing RA. Regarding early RA diagnosis, anti-MCV at the cut-off point was > 1.2 mol/l with 70% sensitivity and 100% specificity. For diagnosis of late RA, the cut-off point was > 1.2 mol/l, with 93.3% sensitivity and 100% specificity, whereas the overall diagnostic accuracy was 96.3%. In this study, patients with positive anti-CCP had a marginally higher level of anti-MCV compared to those with negative anti-CCP (1.64 ± 0.28 vs. 1.48 ± 0.73 mol/l; P= 0.29). We concluded that serum levels of Anti-MCV can be used as a diagnostic test in RA. The increased serum levels of Anti-MCV, demonstrated the importance of Anti-MCV as an independent serum marker in predicting the outcome of RA.
类风湿关节炎（RA）是一种自身免疫性疾病，其特征是针对瓜氨酸化抗原的自身抗体。抗环瓜氨酸肽（Anti-CCP）检测常用于诊断类风湿关节炎，而抗突变型瓜氨酸波形蛋白（Anti-MCV）是另一种与突变型瓜氨酸化波形蛋白反应的抗瓜氨酸化抗体。因此，我们旨在评估抗 MCV 抗体在 RA 患者中的诊断价值及其与疾病活动度的关系。本研究纳入了 60 例 RA 患者和 25 名正常对照。采用疾病活动评分（DAS-28）评估 RA 患者的疾病活动度。采用 ELISA 法检测患者和对照组的抗 MCV 和抗 CCP。RA 患者的抗 MCV 水平明显高于对照组（1.56±0.56 与 1.20±0.19μmol/L；P＜0.001）。抗 MCV 截断值＞1.2μmol/L 时，诊断 RA 的敏感度为 76%，特异度为 100%，总诊断准确性为 83.2%。对于早期 RA 的诊断，截断值＞1.2μmol/L 时，敏感度为 70%，特异度为 100%。对于晚期 RA 的诊断，截断值＞1.2μmol/L 时，敏感度为 93.3%，特异度为 100%，总诊断准确性为 96.3%。在本研究中，抗 CCP 阳性患者的抗 MCV 水平略高于抗 CCP 阴性患者（1.64±0.28 与 1.48±0.73μmol/L；P=0.29）。我们得出结论，血清抗 MCV 水平可作为 RA 的诊断试验。血清抗 MCV 水平升高表明其作为独立的血清标志物在预测 RA 结局方面具有重要意义。
