Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, No. 1 Shida Road, Limin Development Zone, Harbin, 150025, Heilongjiang Province, People's Republic of China.
Ecotoxicology. 2022 Nov;31(9):1356-1368. doi: 10.1007/s10646-022-02595-7. Epub 2022 Oct 8.
Saline-alkalisation of the soil environment and microorganism is a global challenge. However, relevant studies on the effects of saline-alkali stress on soil bacterial communities are limited. In this study, we investigated the effects of saline-alkali stress on the carbon source metabolic utilisation of the microbial community, bacterial diversity, and composition in soil using Biolog Ecoplate and 16S rRNA gene amplicon sequencing. Biolog Ecoplate results showed that saline-alkali stress decreased the metabolic activity and functional diversity, and changed the utilisation characteristics of carbon sources in soil microorganisms. Particularly, high level of saline-alkali stress significantly decreased the utilisation of carbohydrates and amino acids carbon sources. The results of 16S rRNA gene amplicon sequencing showed that high level of saline-alkali stress significantly reduced the diversity of soil bacterial communities. In addition, high level of saline-alkali stress significantly decreased the relative abundances of some key bacterial taxa, such as Gemmatimonas, Sphingomonas, and Bradyrhizobium. Furthermore, as saline-alkali content increased, the soil catalase, protease, urease, and sucrase activities also significantly decreased. Collectively, these results provide new insight for studies on the changes in the soil bacterial community and soil enzyme activity under saline-alkali stress.
土壤环境和微生物的盐碱性化是一个全球性的挑战。然而，关于盐碱性胁迫对土壤细菌群落影响的相关研究还很有限。本研究采用 Biolog Ecoplate 和 16S rRNA 基因扩增子测序技术，研究了盐碱性胁迫对土壤微生物群落碳源代谢利用、细菌多样性和组成的影响。Biolog Ecoplate 结果表明，盐碱性胁迫降低了土壤微生物的代谢活性和功能多样性，并改变了其对碳源的利用特性。特别是，高盐碱性胁迫显著降低了碳水化合物和氨基酸碳源的利用。16S rRNA 基因扩增子测序结果表明，高盐碱性胁迫显著降低了土壤细菌群落的多样性。此外，高盐碱性胁迫还显著降低了 Gemmatimonas、Sphingomonas 和 Bradyrhizobium 等一些关键细菌类群的相对丰度。此外，随着盐碱性含量的增加，土壤过氧化氢酶、蛋白酶、脲酶和蔗糖酶活性也显著降低。总之，这些结果为研究盐碱性胁迫下土壤细菌群落和土壤酶活性的变化提供了新的见解。
