Devi L, Douglass J, Herbert E
Brain Res. 1987 Jul;388(2):173-6. doi: 10.1016/s0006-8993(87)80011-2.
We have developed a RNA-RNA solution hybridization assay to quantitate the mRNA coding for prodynorphin precursor. This assay is extremely sensitive and highly specific. Using this assay we have measured the prodynorphin mRNA in various brain regions and reproductive tissues of rat. When we compared the distribution of prodynorphin mRNA with the dynorphin related peptides in these tissues, we found a general parallelism and a few noteworthy exceptions.
我们开发了一种RNA-RNA溶液杂交试验，用于定量编码强啡肽原前体的mRNA。该试验极其灵敏且高度特异。利用此试验，我们测定了大鼠不同脑区和生殖组织中的强啡肽原mRNA。当我们比较这些组织中强啡肽原mRNA的分布与强啡肽相关肽的分布时，发现了总体的平行关系以及一些值得注意的例外情况。
