Team of Microbiology and Health, Laboratory of Chemistry-Biology Applied to the Environment, Faculty of Sciences, Moulay Ismail University, 11201 Zitoune Meknes BP, Morocco.
Team of Microbiology and Health, Laboratory of Chemistry-Biology Applied to the Environment, Faculty of Sciences, Moulay Ismail University, 11201 Zitoune Meknes BP, Morocco.
Int J Food Microbiol. 2022 Dec 16;383:109960. doi: 10.1016/j.ijfoodmicro.2022.109960. Epub 2022 Oct 3.
In this work, the chemical composition of Thymus zygis subsp. gracilis collected from Ifrane, Morocco, along with the evaluation of the antibacterial, anti-biofilm of Listeria monocytogenes activities, larvicidal effect against L3 larvae of Anisakis, and antioxidant properties of its essential oil (TZG-EO), are reported. GC-MS and GC-FID analyses highlighted the presence of 84 volatile components and strong bactericidal and anti-biofilm activities against L. monocytogenes at a concentration of 0.02 % were demonstrated. Also, larvicidal effect against Anisakis larvae at concentrations of 0.01 and 0.005 % was attained leading to the death of all tested larvae within 4 h. The in situ antibacterial activity of TZG-EO (0.01 and 0.005 %) in smoked fish showed high efficiency against L. monocytogenes growth. TZG-EO could be used as potential antibacterial and larvicidal agents for fighting against foodborne pathogens and extending shelf life of food products.
本工作研究了来自摩洛哥伊夫兰的野生细叶香桃木亚种的化学成分，并评估了其精油（TZG-EO）的抗菌、抗李斯特菌生物膜、杀旋毛虫幼虫活性和抗氧化特性。GC-MS 和 GC-FID 分析表明，该精油含有 84 种挥发性成分，对李斯特菌的杀菌和抗生物膜活性在 0.02%的浓度下表现出较强的活性。此外，TZG-EO 在 0.01%和 0.005%的浓度下对旋毛虫幼虫也表现出杀幼虫活性，所有测试幼虫在 4 小时内全部死亡。TZG-EO（0.01%和 0.005%）在熏鱼中的原位抗菌活性对李斯特菌的生长表现出高效性。TZG-EO 可作为潜在的抗菌和杀幼虫剂，用于对抗食源性病原体，延长食品的保质期。
