Department of Human Nutrition and Hospitality Management, College of Human Environmental Sciences, The University of Alabama, Tuscaloosa, AL, 35487, USA.
Department of Chemical and Biological Engineering, College of Engineering, The University of Alabama, Tuscaloosa, AL, 35487, USA.
Biochem Biophys Res Commun. 2022 Dec 3;632:173-180. doi: 10.1016/j.bbrc.2022.09.084. Epub 2022 Sep 29.
The presence of circulating cancer cells in the bloodstream is positively correlated with metastasis. We hypothesize that fluid shear stress (FSS) occurring during circulation alters mitochondrial function, enhancing metastatic behaviors of cancer cells. MCF7 and MDA-MB-231 human breast cancer cells subjected to FSS exponentially increased proliferation. Notably, FSS-treated cells consumed more oxygen but were resistant to uncoupler-mediated ATP loss. We found that exposure to FSS downregulated the FF ATP synthase c-subunit and overexpression of the c-subunit arrested cancer cell migration. Approaches that regulate c-subunit abundance may reduce the likelihood of breast cancer metastasis.
循环肿瘤细胞在血液中的存在与转移呈正相关。我们假设，在循环过程中发生的流体切应力（FSS）改变了线粒体功能，增强了癌细胞的转移行为。MCF7 和 MDA-MB-231 人乳腺癌细胞受到 FSS 的影响，增殖呈指数增长。值得注意的是，FSS 处理的细胞消耗了更多的氧气，但对解偶联剂介导的 ATP 损失有抗性。我们发现，暴露于 FSS 会下调 FF ATP 合酶 c 亚基，而过表达 c 亚基则会阻止癌细胞的迁移。调节 c 亚基丰度的方法可能会降低乳腺癌转移的可能性。
