Peets J M, Pomeranz B
Brain Res. 1987 Jul 28;416(2):301-7. doi: 10.1016/0006-8993(87)90910-3.
We report on the effects of somatic conditioning stimuli on the reflexive nocifensive tail flick electromyograms (TFEMGs) in the rat anesthetized with continuous pentobarbital infusions. Rather than using a reflexive tail movement as a measure of noxious responses, TFEMGs were recorded from the base of the rat's tail as this gave more reliable results in the anesthetized state. In order to demonstrate the compatibility of this model with previous tail flick studies, we demonstrated an inhibition of TFEMGs by intrathecal morphine which was reversed by intravenous naloxone. The TFEMG latencies were then shown to be increased by electroacupuncture. This effect was antagonized by intrathecal naltrexone pretreatment. All of these results under barbiturate anesthesia resembled those observed previously in awake rats. The constant pentobarbital infusion maintained a stable baseline state, as reflected by TFEMG latencies and blood pressure recordings during the prolonged time-course of each experiment (approximately 1 h). We feel that this method of studying electroacupuncture suppression of nocifensive reflexes is valuable for its relative simplicity and reliability. It also has the virtue of avoiding the pitfalls of such studies in awake animals.
我们报告了躯体条件刺激对持续输注戊巴比妥麻醉的大鼠反射性伤害防御性甩尾肌电图（TFEMGs）的影响。与使用反射性尾部运动作为伤害性反应的指标不同，TFEMGs是从大鼠尾巴根部记录的，因为在麻醉状态下这样能得到更可靠的结果。为了证明该模型与之前甩尾研究的兼容性，我们证明了鞘内注射吗啡可抑制TFEMGs，而静脉注射纳洛酮可逆转这种抑制作用。然后发现电针可增加TFEMG潜伏期。鞘内注射纳曲酮预处理可拮抗这种效应。所有这些在巴比妥类麻醉下的结果与之前在清醒大鼠中观察到的结果相似。在每个实验的较长时间过程（约1小时）中，持续输注戊巴比妥维持了稳定的基线状态，这通过TFEMG潜伏期和血压记录得以体现。我们认为这种研究电针抑制伤害防御反射的方法因其相对简单和可靠而具有价值。它还具有避免在清醒动物中进行此类研究时出现的陷阱的优点。
