Tatematsu M, Lee G, Hayes M A, Farber E
Cancer Res. 1987 Sep 1;47(17):4699-705.
Hepatocyte nodules were induced in Fischer 344 rats using the resistant hepatocyte model. Nodules harvested at 5, 24, or 25 weeks after initiation were isolated, diced, and transplanted into the spleen of normal rats and observed for periods up to 104 weeks. In the first experiment, 50% of the animals developed hepatocellular carcinoma, some with invasion and metastasis, by 70 to 104 weeks. In the second experiment, transplants of 5-week nodules grew very slowly and diffusely in the spleen, as did normal liver, but retained at least some of their phenotypic properties. In contrast, transplants of 25-week nodules grew into nodules up to 2.5 cm in diameter by 70 weeks. Two of the larger nodules had smaller nodules within resembling trabecular carcinoma. Transplants from the liver surrounding the 25-week nodules did not grow and produced no nodules by 70 weeks after transplantation. The implications of these observations in the study of progression in hepatocarcinogenesis are discussed briefly.
利用抗性肝细胞模型在Fischer 344大鼠中诱导肝细胞结节形成。在诱导后5周、24周或25周收获结节，将其分离、切碎，然后移植到正常大鼠的脾脏中，并观察长达104周的时间。在第一个实验中，到70至104周时，50%的动物发生了肝细胞癌，有些伴有侵袭和转移。在第二个实验中，5周龄结节的移植组织在脾脏中生长非常缓慢且呈弥漫性生长，与正常肝脏相似，但至少保留了其一些表型特性。相比之下，25周龄结节的移植组织在70周时生长成直径达2.5厘米的结节。其中两个较大的结节内有较小的结节，类似小梁癌。移植25周龄结节周围肝脏的组织在移植后70周没有生长，也没有产生结节。本文简要讨论了这些观察结果在肝癌发生进展研究中的意义。
