Daroodi Zoha, Taheri Parissa, Tarighi Saeed
Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Front Plant Sci. 2022 Sep 23;13:984583. doi: 10.3389/fpls.2022.984583. eCollection 2022.
In this study, efficiency of the endophytic fungal isolate Msh5 was evaluated on promoting tomato plant growth and controlling , the causal agent of early blight in tomatoes. Morphological and molecular (ITS and tub2 sequences) analyses revealed that the fungal isolate, Msh5, was ( Lodha). This beneficial fungus was capable of producing indole-3-acetic acid (IAA), urease, siderophore, extracellular enzymes, and solubilized phosphate. Under laboratory conditions, the Msh5 isolate of inhibited growth in dual culture, volatile and non-volatile metabolites assays. The supernatant of this endophytic fungus was capable of reducing spore germination and altering the hyphal structure of and the spores produced germ tubes showed vacuolization and abnormal structure compared to the control. Also, the effect of on plant growth parameters (such as shoot and root weight and length) and suppressing was investigated via seed inoculation with spores of using 1% sugar, 0.5% carboxymethyl cellulose (CMC) or 0.5% molasses solution as stickers. Colonization of tomato roots by the endophytic fungus resulted in significant increasing plant growth parameters and reduction in the progress of the diseases caused by compared to the controls. Among the different coating materials used as stickers, sugar was found to be the most effective for enhancing plant growth parameters and decreasing the disease progress. Therefore, isolate Msh5 can be suggested as a potential biofertilizer and biocontrol agent for protecting tomato plants against .
在本研究中，对内生真菌分离株Msh5促进番茄植株生长及防治番茄早疫病病原菌的效率进行了评估。形态学和分子（ITS和tub2序列）分析表明，真菌分离株Msh5为（洛达）。这种有益真菌能够产生吲哚 - 3 - 乙酸（IAA）、脲酶、铁载体、胞外酶并溶解磷酸盐。在实验室条件下，在双重培养、挥发性和非挥发性代谢物测定中，Msh5分离株抑制了（病原菌名称未明确写出）的生长。这种内生真菌的上清液能够降低孢子萌发率，并改变（病原菌名称未明确写出）的菌丝结构，与对照相比，所产生的孢子萌发管出现空泡化和结构异常。此外，通过用1%糖、0.5%羧甲基纤维素（CMC）或0.5%糖蜜溶液作为粘着剂，用（病原菌名称未明确写出）的孢子接种种子，研究了（病原菌名称未明确写出）对植物生长参数（如地上部和根部重量及长度）的影响以及对（病原菌名称未明确写出）的抑制作用。与对照相比，内生真菌在番茄根部的定殖导致植物生长参数显著增加，由（病原菌名称未明确写出）引起的病害进展减缓。在所使用的作为粘着剂的不同包衣材料中，发现糖对于提高植物生长参数和降低病害进展最为有效。因此，可建议Msh5分离株作为一种潜在的生物肥料和生物防治剂，用于保护番茄植株免受（病原菌名称未明确写出）侵害。
