Characteristics of NtCCD1-3 from tobacco, and protein engineering of the CCD1 to enhance -ionone production in yeast.

Biosynthesis of -ionone by microbial cell factories has become a promising way to obtain natural -ionone. The catalytic activity of carotenoid cleavage dioxygenase 1 (CCD1) in cleavage of -carotene to -ionone severely limits its biosynthesis. In this study, NtCCD1-3 from with high ability to cleave -carotene was screened. Multiple strategies for improving the -ionone yield in were performed. The results showed that NtCCD1-3 could cleave a variety of caroteniods at the 9,10 (9',10') double bonds and lycopene at the 5,6 (5',6') positions. The insertion site delta for gene was more suitable for enhancing the yield of -ionone, showing 19.1-fold increase compared with the rox1 site. More importantly, mutant K38A of NtCCD1-3 in membrane-bonding domains could greatly promote -ionone production by more than 3-fold. We also found that overexpression of the NADH kinase Pos5 could improve -ionone yield up to 1.5 times. These results may provide valuable references for biosynthesis of -ionone.