Ingram Zoe, Patkar Abha, Oh Dahoon, Zhang Kevin K, Chung Christina, Lin-Cereghino Joan, Lin-Cereghino Geoff P
University of the Pacific.
Pac J Health (Stockt). 2021;4(1). doi: 10.56031/2576-215x.1010.
The yeast (also known as ) has been used for over 30 years to produce thousands of valuable, heterologous proteins, such as insulin to treat diabetes and antibodies to prevent migraine headaches. Despite its success, there are some common, stubborn problems encountered by research scientists when they try to use the yeast to produce their recombinant proteins. In order to provide those working in this field with strategies to overcome these common obstacles, nine experts in protein expression field were interviewed to create a written review and video (https://www.youtube.com/watch?v=Q1oD6k8CdG8). This review describes how each respected scientist addressed a specific challenge, such as identifying high expression strains, improving secretion efficiency and decreasing hyperglycosylation. Their perspective and practical advice can be a tool to help empower others to express challenging proteins in this popular recombinant host.
酵母（也称为 ）已被用于生产数千种有价值的异源蛋白超过30年，比如用于治疗糖尿病的胰岛素和预防偏头痛的抗体。尽管取得了成功，但研究科学家在尝试使用酵母生产重组蛋白时，仍会遇到一些常见且棘手的问题。为了给该领域的工作人员提供克服这些常见障碍的策略，我们采访了9位蛋白质表达领域的专家，以撰写一篇书面综述并制作了一个视频（https://www.youtube.com/watch?v=Q1oD6k8CdG8）。这篇综述描述了每位备受尊敬的科学家是如何应对特定挑战的，例如识别高表达菌株、提高分泌效率和减少高糖基化。他们的观点和实用建议可以成为一种工具，帮助其他人在这种流行的重组宿主中表达具有挑战性的蛋白质。
