Wang Hongjing, Zheng Ziming, Wu Zhijing, Song Mengzi, Yu Zhang, Wang Kaiping
Wuhan Fourth Hospital, Wuhan, China.
Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Nat Prod Res. 2023 Aug-Sep;37(16):2697-2703. doi: 10.1080/14786419.2022.2130916. Epub 2022 Oct 10.
Lindl. has been long used in China as a functional food and traditional Chinese medicine and polysaccharides from Lindl. (DOP) exhibited extensive bioactivities. However, studies on the structure-activity relationship of DOP are rarely reported. Here, two polysaccharides named DOP-1 and DOP-2 were obtained, which differed in the ratio of monosaccharide composition and molecular weight. Structural characteristics were elucidated by spectral and chemical analysis. The main structures of DOPs were the linkage of β-(1→4)-D-Man, with some attached 2-- or 3--acetylated groups. Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content. Our results provide a more accurate basis for the application of DOPs in the pharmaceutical and food industries.
在中国，[植物名称未给出，原文Lindl.有误，推测可能是某种植物]长期以来一直被用作功能性食品和传统中药，并且从[植物名称未给出，原文Lindl.有误，推测可能是某种植物]中提取的多糖（DOP）具有广泛的生物活性。然而，关于DOP结构-活性关系的研究鲜有报道。在此，获得了两种名为DOP-1和DOP-2的多糖，它们在单糖组成比例和分子量上有所不同。通过光谱和化学分析阐明了其结构特征。DOPs的主要结构是β-(1→4)-D-甘露糖的连接，并带有一些连接的2-或3-乙酰化基团。此外，DOP-1和DOP-2的DPPH、羟基和超氧阴离子自由基清除试验表明，DOP-2表现出更高的抗氧化活性，这可能与其较低的分子量、较高的甘露糖比例、较低的乙酰化程度以及较高的酚类含量有关。我们的结果为DOPs在制药和食品工业中的应用提供了更准确的依据。
