Tianjin Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, P. R. China.
Shijiazhuang Yiling Pharmaceutical Co., Ltd., No. 238 Tianshan Road, Hi Tech Industrial Development Zone, Shijiazhuang, 050035, P. R. China.
Chem Biodivers. 2021 May;18(5):e2001023. doi: 10.1002/cbdv.202001023. Epub 2021 Apr 21.
In this study, Dendrobium officinale polysaccharide (named DOPS-1) was isolated from the stems of Dendrobium officinale by hot-water extraction and purified by using Sephadex G-150 column chromatography. The structural characterization, antioxidant and cytotoxic activity were carried out. Based on the results of HPLC, GC, Congo red experiment, together with periodate oxidation, Smith degradation, SEM, FT-IR, and NMR spectral analysis, it expressed that DOPS-1 was largely composed of mannose, glucose and galacturonic acid in a molar ratio of 3.2 : 1.3 : 1. The molecular weight of DOPS-1 was 1530 kDa and the main chain was composed of (1→4)-β-D-Glcp, (1→4)-β-D-Manp and 2-O-acetyl-(1→4)-β-D-Manp. The measurement results of antioxidant activity showed that DOPS-1 had the strong scavenging activities on hydroxyl radicals, DPPH radicals and superoxide radicals and the high reducing ability in vitro. Moreover, DOPS-1 was cytotoxic to all three human cancer cells of MDA-MB-231, A549 and HepG2.
在这项研究中，从铁皮石斛茎中提取了铁皮石斛多糖（命名为 DOPS-1），并用 Sephadex G-150 柱层析法进行纯化。对其结构特征、抗氧化和细胞毒性活性进行了研究。根据 HPLC、GC、刚果红实验的结果，以及过碘酸盐氧化、Smith 降解、SEM、FT-IR 和 NMR 光谱分析，表明 DOPS-1 主要由甘露糖、葡萄糖和半乳糖醛酸组成，摩尔比为 3.2:1.3:1。DOPS-1 的分子量为 1530 kDa，主链由 (1→4)-β-D-Glcp、(1→4)-β-D-Manp 和 2-O-乙酰基-(1→4)-β-D-Manp 组成。抗氧化活性的测定结果表明，DOPS-1 对羟基自由基、DPPH 自由基和超氧阴离子自由基具有很强的清除活性，具有体外高还原能力。此外，DOPS-1 对三种人癌细胞 MDA-MB-231、A549 和 HepG2 均具有细胞毒性。
